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Literature summary for 7.1.1.2 extracted from
- Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae (1988), Eur. J. Biochem., 176, 377-384.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
NADH | with ubiquinone-6 as acceptor | Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 0.08 mol Fe per mol of flavin after dialysis | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
rotenone-insensitive enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 61.9 | - |
reaction with ubiquinone-6 | Saccharomyces cerevisiae |
| 1671 | - |
reaction with ubiquinone-2 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + ubiquinone | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol | - |
? | |
| NADH + ubiquinone-2 | - |
Saccharomyces cerevisiae | NAD+ + reduced ubiquinol-2 | - |
? | |
| NADH + ubiquinone-6 | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol-6 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 80 | 100 | NADH | with ubiquinone-6 as acceptor, Triton assay medium | Saccharomyces cerevisiae | |
| 500 | 550 | NADH | with ubiquinone-6 as acceptor, mitochondria | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | reaction with ubiquinone-6 is almost constant between pH 4.5 and pH 9.5 | Saccharomyces cerevisiae |
| 6.2 | - |
reaction with ubiquinone-2 | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
activity is irreversibly destroyed below | Saccharomyces cerevisiae |
| 9.5 | - |
activity is irreversibly destroyed above | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains a single molecule of FAD per subunit | Saccharomyces cerevisiae | |
| NADH | - |
Saccharomyces cerevisiae | |
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