Literature summary for 7.1.1.10 extracted from

Battchikova, N.; Aro, E.M.
Cyanobacterial NDH-1 complexes multiplicity in function and subunit composition (2007), Physiol. Plant., 131, 22-32 .

Protein Variants

Protein Variants	Comment	Organism
additional information	in DELTAndhD1 and DELTAndhD1/D2 mutants, the NDH-1L complex is missing but NDH-1M and NDH-1S are abundant in low-CO2 conditions. The DELTAndhD3 and DELTAndhD3/D4 mutants lack NDH-1S but exhibit wild-type levels of NDH-1M at low CO2. In both mutants, the carbon uptake is impaired and more severely in the double mutant that can grow at low CO2 only at elevated pH (pH 8.3 but not 7.5), when other mechanisms of carbon uptake are activated. Deletion of the small hydrophobic NdhL subunit is not essential for the assembly of NDH-1L and NDH-1M. This subunit might be important in supporting a specific conformation of the NDH-1 complexes because the importance of NdhL for the function in CO2 uptake is unambiguously demonstrated	Synechocystis sp. 6803

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	all NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helixes	Thermosynechococcus vestitus	-	-
additional information	all NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helixes	Nostoc sp. PCC 7120 = FACHB-418	-	-
additional information	all NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helixes	Synechocystis sp. 7942	-	-
additional information	all NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helixes	Synechocystis sp. 7002	-	-
additional information	in Synechocystis sp. and in Anacystis nidulans, subunits NdhJ and NdhK are found in both the plasma membrane and the thylakoid membrane. All NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helices	Synechococcus elongatus PCC 7942 = FACHB-805	-	-
additional information	in Synechocystis sp. and in Anacystis nidulans, subunits NdhJ and NdhK are found in both the plasma membrane and the thylakoid membrane. All NdhF, NdhD proteins and NdhB contain 12-17 transmembrane helixes	Synechocystis sp. 6803	-	-
plasma membrane	-	Synechococcus elongatus PCC 7942 = FACHB-805	5886	-
plasma membrane	-	Synechocystis sp. 6803	5886	-
plasma membrane	Anabaena sp. PCC 7120, the NDH-1 complex is localized to the plasma membrane only	Nostoc sp. PCC 7120 = FACHB-418	5886	-
thylakoid membrane	-	Synechococcus elongatus PCC 7942 = FACHB-805	42651	-
thylakoid membrane	-	Thermosynechococcus vestitus	42651	-
thylakoid membrane	-	Synechocystis sp. 7942	42651	-
thylakoid membrane	-	Synechocystis sp. 7002	42651	-
thylakoid membrane	-	Synechocystis sp. 6803	42651	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Synechococcus elongatus PCC 7942 = FACHB-805	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Thermosynechococcus vestitus	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Nostoc sp. PCC 7120 = FACHB-418	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Synechocystis sp. 7942	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Synechocystis sp. 7002	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	Synechocystis sp. 6803	-	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Nostoc sp. PCC 7120 = FACHB-418	-	-	-
Synechococcus elongatus PCC 7942 = FACHB-805	-	-	-
Synechocystis sp. 6803	-	-	-
Synechocystis sp. 7002	-	-	-
Synechocystis sp. 7942	-	-	-
Thermosynechococcus vestitus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
in the NDH-1 complex, the interactions between the connecting and membrane core domains seem to be strong enough to keep them together during solubilization	Thermosynechococcus vestitus
in the NDH-1 complex, the interactions between the connecting and membrane core domains seem to be strong enough to keep them together during solubilization	Synechocystis sp. 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Synechococcus elongatus PCC 7942 = FACHB-805	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Thermosynechococcus vestitus	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Nostoc sp. PCC 7120 = FACHB-418	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Synechocystis sp. 7942	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Synechocystis sp. 7002	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?
2 reduced ferredoxin [iron-sulfur] cluster + plastoquinone + 6 H+[side 1]	-	Synechocystis sp. 6803	2 oxidized ferredoxin [iron-sulfur] cluster + plastoquinol + 7 H+[side 2]	-	?

Synonyms

Synonyms	Comment	Organism
cyanobacterial NDH-1 complex	-	Synechococcus elongatus PCC 7942 = FACHB-805
cyanobacterial NDH-1 complex	-	Thermosynechococcus vestitus
cyanobacterial NDH-1 complex	-	Nostoc sp. PCC 7120 = FACHB-418
cyanobacterial NDH-1 complex	-	Synechocystis sp. 7942
cyanobacterial NDH-1 complex	-	Synechocystis sp. 7002
cyanobacterial NDH-1 complex	-	Synechocystis sp. 6803

Cofactor

Cofactor	Comment	Organism
Fe-S cluster	presence of several iron-sulfur clusters	Synechococcus elongatus PCC 7942 = FACHB-805
Fe-S cluster	presence of several iron-sulfur clusters	Thermosynechococcus vestitus
Fe-S cluster	presence of several iron-sulfur clusters	Nostoc sp. PCC 7120 = FACHB-418
Fe-S cluster	presence of several iron-sulfur clusters	Synechocystis sp. 7942
Fe-S cluster	presence of several iron-sulfur clusters	Synechocystis sp. 7002
Fe-S cluster	presence of several iron-sulfur clusters	Synechocystis sp. 6803
Ferredoxin	-	Synechococcus elongatus PCC 7942 = FACHB-805
Ferredoxin	-	Thermosynechococcus vestitus
Ferredoxin	-	Nostoc sp. PCC 7120 = FACHB-418
Ferredoxin	-	Synechocystis sp. 7942
Ferredoxin	-	Synechocystis sp. 7002
Ferredoxin	-	Synechocystis sp. 6803
FMN	-	Synechococcus elongatus PCC 7942 = FACHB-805
FMN	-	Thermosynechococcus vestitus
FMN	-	Nostoc sp. PCC 7120 = FACHB-418
FMN	-	Synechocystis sp. 7942
FMN	-	Synechocystis sp. 7002
FMN	-	Synechocystis sp. 6803
plastoquinone	-	Synechococcus elongatus PCC 7942 = FACHB-805
plastoquinone	-	Thermosynechococcus vestitus
plastoquinone	-	Nostoc sp. PCC 7120 = FACHB-418
plastoquinone	-	Synechocystis sp. 7942
plastoquinone	-	Synechocystis sp. 7002
plastoquinone	-	Synechocystis sp. 6803

General Information

General Information	Comment	Organism
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Synechococcus elongatus PCC 7942 = FACHB-805
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Thermosynechococcus vestitus
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Nostoc sp. PCC 7120 = FACHB-418
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Synechocystis sp. 7942
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Synechocystis sp. 7002
evolution	the ndhD and ndhF genes belong to a large family and might have appeared by ancient gene duplication events during evolution. NdhD1 and NdhF1 are present in all cyanobacteria sequenced so far, but the occurrence of other NdhD and NdhF genes varies among cyanobacteria. The ndhD1/ndhD2 and ndhF1 are related to chloroplast ndhD and ndhF genes, respectively, whereas the ndhD3/ndhD4 and ndhF3/ndhF4 types of genes are specific to cyanobacteria	Synechocystis sp. 6803
malfunction	inactivation of ndhB or ndhK results in a high-CO2-requiring phenotype. In cells of the ndhD1/D2 double mutant grown in high CO2, the cyclic electron flow around PSI is strongly impaired and the respiration activity is also low. But cells of this mutant grown at low CO2 show PSI cyclic electron flow similar to wild-type. The donation of electron from NADPH to plastoquinone occurs in thylakoid membranes of wild-type Synechocystis 6803 but is impaired in those of the NdhB-defective mutant	Synechocystis sp. 6803
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain	Synechococcus elongatus PCC 7942 = FACHB-805
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain	Thermosynechococcus vestitus
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain	Synechocystis sp. 7942
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain	Synechocystis sp. 7002
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain	Synechocystis sp. 6803
metabolism	reverse genetics and proteomics studies focussing on the structural and functional differences of the two types of cyanobacterial NDH-1 complexes: NDH-1L, important for respiration and PSI cyclic electron flow, and NDH-1MS, the low-CO2 inducible complex participating in CO2 uptake, overview. The NDH-1 complexes in cyanobacteria share a common NDH-1M core complex and differ in the composition of the distal membrane domain composed of specific NdhD and NdhF proteins, which in complexes involved in CO2 uptake is further associated with the hydrophilic carbon uptake (CUP) domain. In Anabaena sp. PCC 7120, the NDH-1 complex is localized to the plasma membrane only	Nostoc sp. PCC 7120 = FACHB-418
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Synechococcus elongatus PCC 7942 = FACHB-805
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Thermosynechococcus vestitus
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Nostoc sp. PCC 7120 = FACHB-418
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Synechocystis sp. 7942
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Synechocystis sp. 7002
additional information	soluble and membrane-type subunits, composition of the NDH-1 complex types in cyanobacteria: Ndh-1L, Ndh-1M, and Ndh-1MS, complex structure and interactions, detailed overview	Synechocystis sp. 6803
physiological function	NDH-1/complex I is a multisubunit enzyme with the following characteristic features: the presence of flavin mononucleotide (FMN) and several iron-sulfur (Fe-S) clusters, sensitivity to rotenone or piericidin A, and an L-shaped structure with a membrane domain and a perpendicular peripheral domain composed of hydrophobic and hydrophilic subunits. In cyanobacteria, the NDH-1 complexes are involved in inorganic carbon-concentrating mechanisms, which are vitally important for aquatic phototrophs under carbon limitation in order to compensate for the relatively low affinity of their Rubisco for CO2. NDH-1 participates in cellular respiration and PSI cyclic electron flow in cyanobacteria, and the NdhB subunit is essential for both functions	Synechococcus elongatus PCC 7942 = FACHB-805
physiological function	NDH-1/complex I is a multisubunit enzyme with the following characteristic features: the presence of flavin mononucleotide (FMN) and several iron-sulfur (Fe-S) clusters, sensitivity to rotenone or piericidin A, and an L-shaped structure with a membrane domain and a perpendicular peripheral domain composed of hydrophobic and hydrophilic subunits. In cyanobacteria, the NDH-1 complexes are involved in inorganic carbon-concentrating mechanisms, which are vitally important for aquatic phototrophs under carbon limitation in order to compensate for the relatively low affinity of their Rubisco for CO2. NDH-1 participates in cellular respiration and PSI cyclic electron flow in cyanobacteria, and the NdhB subunit is essential for both functions	Thermosynechococcus vestitus
physiological function	NDH-1/complex I is a multisubunit enzyme with the following characteristic features: the presence of flavin mononucleotide (FMN) and several iron-sulfur (Fe-S) clusters, sensitivity to rotenone or piericidin A, and an L-shaped structure with a membrane domain and a perpendicular peripheral domain composed of hydrophobic and hydrophilic subunits. In cyanobacteria, the NDH-1 complexes are involved in inorganic carbon-concentrating mechanisms, which are vitally important for aquatic phototrophs under carbon limitation in order to compensate for the relatively low affinity of their Rubisco for CO2. NDH-1 participates in cellular respiration and PSI cyclic electron flow in cyanobacteria, and the NdhB subunit is essential for both functions. NdhD1(D2)/NdhF1 are important for both respiration and cyclic electron flow, whereas NdhD3/NdhF3 are responsible for low-CO2-inducible, high-affinity CO2 uptake system, and NdhD4/NdhF4 is involved in a constitutive, low-affinity CO2 uptake. The roles of NdhD5 and NdhD6 are undetermined	Synechocystis sp. 7942
physiological function	NDH-1/complex I is a multisubunit enzyme with the following characteristic features: the presence of flavin mononucleotide (FMN) and several iron-sulfur (Fe-S) clusters, sensitivity to rotenone or piericidin A, and an L-shaped structure with a membrane domain and a perpendicular peripheral domain composed of hydrophobic and hydrophilic subunits. In cyanobacteria, the NDH-1 complexes are involved in inorganic carbon-concentrating mechanisms, which are vitally important for aquatic phototrophs under carbon limitation in order to compensate for the relatively low affinity of their Rubisco for CO2. NDH-1 participates in cellular respiration and PSI cyclic electron flow in cyanobacteria, and the NdhB subunit is essential for both functions. NdhD1(D2)/NdhF1 are important for both respiration and cyclic electron flow, whereas NdhD3/NdhF3 are responsible for low-CO2-inducible, high-affinity CO2 uptake system, and NdhD4/NdhF4 is involved in a constitutive, low-affinity CO2 uptake. The roles of NdhD5 and NdhD6 are undetermined	Synechocystis sp. 7002
physiological function	NDH-1/complex I is a multisubunit enzyme with the following characteristic features: the presence of flavin mononucleotide (FMN) and several iron-sulfur (Fe-S) clusters, sensitivity to rotenone or piericidin A, and an L-shaped structure with a membrane domain and a perpendicular peripheral domain composed of hydrophobic and hydrophilic subunits. In cyanobacteria, the NDH-1 complexes are involved in inorganic carbon-concentrating mechanisms, which are vitally important for aquatic phototrophs under carbon limitation in order to compensate for the relatively low affinity of their Rubisco for CO2. NDH-1 participates in cellular respiration and PSI cyclic electron flow in cyanobacteria, and the NdhB subunit is essential for both functions. Subunit NdhL plays an important role in CO2 uptake and is part of the Synechocystis NDH-1 complex. In contrast to the NdhB, NdhL and NdhK subunits, the ndhH gene is vital to the survival of Synechocystis 6803 even under high-CO2 growth conditions. NDH-1MS is also involved in cyclic electron flow	Synechocystis sp. 6803