Literature summary for 7.1.1.1 extracted from

Karlsson, J.; Althage, M.; Rydstrom, J.
Roles of individual amino acids in helix 14 of the membrane domain of proton-translocating transhydrogenase from Escherichia coli as deduced from cysteine mutagenesis (2003), Biochemistry, 42, 6575-6581.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli
expressed in Escherichia coli as cysteine free variant	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
A246C	reverse activity stronger affected than cyclic activity	Escherichia coli
A253C	reverse activity stronger affected than cyclic activity	Escherichia coli
D238C	reverse activity stronger affected than cyclic activity	Escherichia coli
G245C	24% of reverse activity	Escherichia coli
G245L	52% of cyclic activity	Escherichia coli
G245L	the mutation leads to a general inhibition of all enzyme activities	Escherichia coli
G249C	40% of reverse activity	Escherichia coli
G249L	48% of cyclic activity	Escherichia coli
G249L	70% of reverse activity	Escherichia coli
G249L	the mutation leads to a general inhibition of all enzyme activities	Escherichia coli
G252C	less than 5% of reverse activity	Escherichia coli
G252L	13% of cyclic activity	Escherichia coli
G252L	the mutation leads to a general inhibition of all enzyme activities	Escherichia coli
I258C	reverse activity stronger affected than cyclic activity	Escherichia coli
L240C	reverse activity stronger affected than cyclic activity	Escherichia coli
L241C	reverse activity stronger affected than cyclic activity	Escherichia coli
L254C	reverse activity stronger affected than cyclic activity	Escherichia coli
L255C	reverse activity stronger affected than cyclic activity	Escherichia coli
M259C	21% of reverse activity, 215% of cyclic activity	Escherichia coli
N238C	reverse activity stronger affected than cyclic activity	Escherichia coli
S250C	strongly increased reverse and cyclic activity	Escherichia coli
S250C	the mutation leads to enhanced activities of all enzyme activities	Escherichia coli
S251C	strongly increased reverse and cyclic activity	Escherichia coli
S251C	the mutation leads to enhanced activities of all enzyme activities	Escherichia coli
S256C	the mutation leads to enhanced activities of all enzyme activities	Escherichia coli
T244C	reverse activity stronger affected than cyclic activity	Escherichia coli
V243C	reverse activity stronger affected than cyclic activity	Escherichia coli
V248C	reverse activity stronger affected than cyclic activity	Escherichia coli
Y257C	reverse activity stronger affected than cyclic activity	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NADH + NADP+	Escherichia coli	links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane	NAD+ + NADPH	-	r
NADPH + NAD+ + H+[side 1]	Escherichia coli	-	NADP+ + NADH + H+[side 2]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
recombinant protein	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
NADH + NADP+	-	Escherichia coli	NAD+ + NADPH	-	r
NADH + NADP+	links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane	Escherichia coli	NAD+ + NADPH	-	r
NADPH + NAD+ + H+[side 1]	-	Escherichia coli	NADP+ + NADH + H+[side 2]	-	?

Subunits

Subunits	Comment	Organism
dimer	monomer composed of three domians: dI, dII, and dIII	Escherichia coli

Synonyms

Synonyms	Comment	Organism
proton-translocating nicotinamide nucleotide transhydrogenase	-	Escherichia coli
proton-translocating transhydrogenase	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)+	-	Escherichia coli
NAD(P)H	-	Escherichia coli