Literature summary for 7.1.1.1 extracted from

Bergkvist, A.; Johansson, C.; Johansson, T.; Rydstr�m, J.; Karlsson, g.
Interactions of the NADP(H)-binding domain III of proton-translocating transhydrogenase from Escherichia coli with NADP(H) and the NAD(H)-binding domain I studied by NMR and site-directed mutagenesis (2000), Biochemistry, 39, 12595-12605.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of domain I in Escherichia coli	Rhodospirillum rubrum
expression of wild-type domain III, T393C, R425C, G430C and A432C mutant domain III in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
A432C	mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 150% higher reaction rate than wild-type domain III/R. rubrum domain I mixture	Escherichia coli
G430C	mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 850% higher reaction rate than wild-type domain III/R. rubrum domain I mixture	Escherichia coli
R425C	mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 425% higher reaction rate than wild-type domain III/R. rubrum domain I mixture	Escherichia coli
T393C	mutation in domain III, reverse reaction in the presence of domain I from R. rubrum, 175% higher reaction rate than wild-type domain III/R. rubrum domain I mixture	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Rhodospirillum rubrum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant domain I	Rhodospirillum rubrum
recombinant wild-type, T393C, R425C, G430C and A432C mutant domain III	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.0667	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of wild-type domain III/R. rubrum domain I mixture	Escherichia coli
0.1	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of A432C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.117	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of D393C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.167	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of H345C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.167	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of R350C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.283	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of K424C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.283	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of R425C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.55	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of A348C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.567	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of G430C mutant domain III/R. rubrum domain I mixture	Escherichia coli
0.917	-	reduced acetylpyridine adenine dinucleotide	reverse reaction of D392C mutant domain III/R. rubrum domain I mixture	Escherichia coli
10	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of R425C mutant domain III/R. rubrum domain I mixture	Escherichia coli
11.7	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of G430C mutant domain III/R. rubrum domain I mixture	Escherichia coli
15	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of D392C mutant domain III/R. rubrum domain I mixture	Escherichia coli
20.8	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of H345C mutant domain III/R. rubrum domain I mixture	Escherichia coli
33.3	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of R350C mutant domain III/R. rubrum domain I mixture	Escherichia coli
36.7	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of K424C mutant domain III/R. rubrum domain I mixture	Escherichia coli
40	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of D393C mutant domain III/R. rubrum domain I mixture	Escherichia coli
51.7	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of A432C mutant domain III/R. rubrum domain I mixture	Escherichia coli
55	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of A348C mutant domain III/R. rubrum domain I mixture	Escherichia coli
81.7	-	reduced acetylpyridine adenine dinucleotide	cyclic reaction of wild-type domain III/R. rubrum domain I mixture	Escherichia coli

Cofactor

Cofactor	Comment	Organism
NAD+	-	Escherichia coli
NAD+	-	Rhodospirillum rubrum
NADH	-	Escherichia coli
NADH	-	Rhodospirillum rubrum
NADP+	-	Escherichia coli
NADP+	-	Rhodospirillum rubrum
NADPH	-	Escherichia coli
NADPH	-	Rhodospirillum rubrum