Literature summary for 7.1.1.1 extracted from

Hu, X.; Zhang, J.; Fjellstrom, O.; Bizouarn, T.; Rydstrom, J.
Site-directed mutagenesis of charged and potentially proton-carrying residues in the beta subunit of the proton-translocating nicotinamide nucleotide transhydrogenase from Escherichia coli. Characterization of the beta H91, beta D392, and beta K424 mutants (1999), Biochemistry, 38, 1652-1658.

Search for more literature for 7.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
His-tagged H91E mutant enzyme expressed in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D401E	mutation in beta subunit	Escherichia coli
D401G	mutation in beta subunit	Escherichia coli
D401V	mutation in beta subunit	Escherichia coli
E413D	mutation in beta subunit	Escherichia coli
E413G	mutation in beta subunit	Escherichia coli
E413V	mutation in beta subunit	Escherichia coli
H91E	mutation in beta subunit	Escherichia coli
H91K	mutation in beta subunit	Escherichia coli
K416G	mutation in beta subunit	Escherichia coli
K424G	mutation in beta subunit	Escherichia coli
K424R	mutation in beta subunit	Escherichia coli
K452D	mutation in beta subunit	Escherichia coli
K452G	mutation in beta subunit	Escherichia coli
additional information	properties of a variety of mutant enzymes containing modified conserved and semiconserved basic and acidic residues in the beta subunit	Escherichia coli
R425E	mutation in beta subunit	Escherichia coli
R425G	mutation in beta subunit	Escherichia coli
R425K	mutation in beta subunit	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged H91E mutant enzyme	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	H91E mutant enzyme, optima for forward reaction below pH 5.5, 7% of wild-type enzyme activity at pH 6.0	Escherichia coli
6	-	reverse reaction catalyzed by H91E mutant enzyme, 20% of wild-type enzyme activity	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Escherichia coli
NADH	-	Escherichia coli
NADP+	-	Escherichia coli
NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)