Cloned (Comment) | Organism |
---|---|
- |
Rhodospirillum rubrum |
expression of cysteine mutants A348C, A390C, K424C, and R425C in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A348C | mutation introduced into a cysteine-free mutant enzyme, mutant shows markedly reduced activity | Escherichia coli |
A390C | mutation introduced into a cysteine-free mutant enzyme | Escherichia coli |
K424C | mutation introduced into a cysteine-free mutant enzyme, mutant shows markedly reduced activity | Escherichia coli |
R425C | mutation introduced into a cysteine-free mutant enzyme, mutant shows markedly reduced activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(4-maleimidoanilino)-naphthalene-6-sulfonic acid | 0.004 mM, 2 h incubation, 75% inhibition of reverse reaction catalyzed by A348C mutant enzyme, 95% inhibition of A390C mutant enzyme after 1 h, 90% inhibition of K424C mutant enzyme after 1 h, 55% inhibition of R425C mutant enzyme after 1h | Escherichia coli | |
3-Aminopyridine adenine dinucleotide phosphate | competitive vs. NADP(H) | Escherichia coli | |
N-ethylmaleimide | 0.2 mM, 57% inhibition of reverse reaction in A348C mutant enzyme, 60% inhibition of K424C mutant enzyme, more than 95% inhibition of A390C mutant enzyme after 1h, R425C mutant enzyme is not inhibited | Escherichia coli | |
N-ethylmaleimide | - |
Rhodospirillum rubrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADPH | cysteine-free mutant enzyme | Escherichia coli | |
0.023 | - |
NADPH | A348C mutation introduced into cysteine-free mutant enzyme | Escherichia coli | |
0.033 | - |
NADPH | A390C mutation introduced into cysteine-free mutant enzyme | Escherichia coli | |
0.04 | - |
NADPH | K424C mutation introduced into cysteine-free mutant enzyme | Escherichia coli | |
0.12 | - |
NADPH | R425C mutation introduced into cysteine-free mutant enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
tetramer | domain I, i.e. alpha1 to alpha404, and III i.e. beta260 to beta462, are exposed to the cytosol and contain the binding sites for NAD(H) and NAD(P)H, respectively, domain II, i.e. alpha405 to alpha 510, spans the membrane | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Rhodospirillum rubrum | |
NADH | - |
Escherichia coli | |
NADH | - |
Rhodospirillum rubrum | |
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Rhodospirillum rubrum | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Rhodospirillum rubrum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3-Aminopyridine adenine dinucleotide phosphate | approximate value | Escherichia coli |