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Literature summary for 6.7.1.1 extracted from

  • Waldman, A.; Balskus, E.
    Discovery of a diazo-forming enzyme in cremeomycin biosynthesis (2018), J. Org. Chem., 83, 7539-7546 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 and Tuner cells Streptomyces lividans

Protein Variants

Protein Variants Comment Organism
E352A inactive Streptomyces lividans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite Streptomyces lividans
-
AMP + diphosphate + cremeomycin + H2O
-
?
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite Streptomyces lividans TK64
-
AMP + diphosphate + cremeomycin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-
Streptomyces lividans TK64
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Streptomyces lividans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite
-
Streptomyces lividans AMP + diphosphate + cremeomycin + H2O
-
?
ATP + 3-amino-2-hydroxy-4-methoxybenzoate + nitrite
-
Streptomyces lividans TK64 AMP + diphosphate + cremeomycin + H2O
-
?

Synonyms

Synonyms Comment Organism
creM
-
Streptomyces lividans

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Streptomyces lividans