BRENDA - Enzyme Database show
show all sequences of 6.6.1.2

Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization

Romao, C.V.; Ladakis, D.; Lobo, S.A.; Carrondo, M.A.; Brindley, A.A.; Deery, E.; Matias, P.M.; Pickersgill, R.W.; Saraiva, L.M.; Warren, M.J.; Proc. Natl. Acad. Sci. USA 108, 97-102 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Desulfovibrio vulgaris
-
Salmonella enterica
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Desulfovibrio vulgaris
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Salmonella enterica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Desulfovibrio vulgaris
-
-
periplasm
-
Salmonella enterica
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Desulfovibrio vulgaris
-
-
-
Salmonella enterica
-
-
-
Purification (Commentary)
Commentary
Organism
-
Desulfovibrio vulgaris
-
Salmonella enterica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the protein binds cobalt via residues His154, Glu184, and His216
716791
Desulfovibrio vulgaris
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
-
Desulfovibrio vulgaris
tetramer
-
Salmonella enterica
Cofactor
Cofactor
Commentary
Organism
Structure
heme b
-
Desulfovibrio vulgaris
heme b
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
-
Salmonella enterica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme b
-
Desulfovibrio vulgaris
heme b
-
Salmonella enterica
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Desulfovibrio vulgaris
hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate
Salmonella enterica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
-
Desulfovibrio vulgaris
-
-
periplasm
-
Salmonella enterica
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Desulfovibrio vulgaris
-
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the protein binds cobalt via residues His154, Glu184, and His216
716791
Desulfovibrio vulgaris
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Desulfovibrio vulgaris
tetramer
-
Salmonella enterica
Other publictions for EC 6.6.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748769
Moore
Towards a cell factory for vi ...
Bacillus megaterium, Bacillus megaterium DSM 319
New Biotechnol.
31
553-561
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
716791
Romao
Evolution in a family of chela ...
Desulfovibrio vulgaris, Salmonella enterica
Proc. Natl. Acad. Sci. USA
108
97-102
2011
-
-
2
2
-
-
-
-
2
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699853
Lundqvist
The AAA(+) motor complex of su ...
Brucella melitensis
J. Struct. Biol.
167
227-234
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15270
Debussche
Biosynthesis of the corrin mac ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
175
7430-7440
1993
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653078
Debussche
Assay, purification, and chara ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
174
7445-7451
1992
-
-
-
-
-
-
10
3
-
1
4
1
-
3
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
3
-
1
4
1
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-