Literature summary for 6.6.1.1 extracted from

Adams, N.B.; Marklew, C.J.; Qian, P.; Brindley, A.A.; Davison, P.A.; Bullough, P.A.; Hunter, C.N.
Structural and functional consequences of removing the N-terminal domain from the magnesium chelatase ChlH subunit of Thermosynechococcus elongatus (2014), Biochem. J., 464, 315-322 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
GUN4 protein	stimulates enzyme activity significantly especially at low Mg2+ concentrations	Thermosynechococcus vestitus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta 2 pLysS cells	Thermosynechococcus vestitus

Protein Variants

Protein Variants	Comment	Organism
DELTAN159ChlH	the mutant retains the ability to bind protoporphyrin and the Gun4 enhancer protein, although the addition of I and D subunits yields an extremely impaired active enzyme complex	Thermosynechococcus vestitus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	Thermosynechococcus vestitus	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal affinity chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration	Thermosynechococcus vestitus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	-	Thermosynechococcus vestitus	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	1 * 150000 + 1 * 70000 + 1 * 40000, SDS-PAGE	Thermosynechococcus vestitus

Synonyms

Synonyms	Comment	Organism
ChlH	subunit	Thermosynechococcus vestitus
MGCH	-	Thermosynechococcus vestitus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Thermosynechococcus vestitus

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Release 2023.1 (January 2023)