Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.6.1.1 extracted from

  • Lundqvist, J.; Braumann, I.; Kurowska, M.; Mueller, A.H.; Hansson, M.
    Catalytic turnover triggers exchange of subunits of the magnesium chelatase AAA+ motor unit (2013), J. Biol. Chem., 288, 24012-24019.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Rhodobacter capsulatus

Protein Variants

Protein Variants Comment Organism
D207N BchI mutant forms a complex with wild-type BchD. Mutant shows 7.2% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
L111F BchI mutant forms a complex with wild-type BchD. Mutant shows 5.5% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
additional information mixing experiments with modified and wild-type BchI in various combinations show that an exchange of BchI subunits in magnesium chelatase occurs during the catalytic cycle, indicating that a dissociation of the complex may be part of the reaction mechanism Rhodobacter capsulatus
R289K BchI mutant forms a complex with wild-type BchD. Mutant shows 10.9% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity Rhodobacter capsulatus
V113G BchI mutant forms a complex with wild-type BchD. Mutant shows 25% of wild-type ATPase activity. Mutant shows magnesium chelatase activity. Magnesium chelatase assays containing a mixture of mutant BchI V113G and wild-type BchI have the effect of stimulating the activity Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deuteroporphyrin IX + Mg2+ + H2O
-
Rhodobacter capsulatus ADP + phosphate + Mg-deuteroporphyrin IX + H+
-
?

Synonyms

Synonyms Comment Organism
BchID
-
Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Rhodobacter capsulatus