Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Rhodobacter capsulatus |
Protein Variants | Comment | Organism |
---|---|---|
D207N | BchI mutant forms a complex with wild-type BchD. Mutant shows 7.2% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity | Rhodobacter capsulatus |
L111F | BchI mutant forms a complex with wild-type BchD. Mutant shows 5.5% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity | Rhodobacter capsulatus |
additional information | mixing experiments with modified and wild-type BchI in various combinations show that an exchange of BchI subunits in magnesium chelatase occurs during the catalytic cycle, indicating that a dissociation of the complex may be part of the reaction mechanism | Rhodobacter capsulatus |
R289K | BchI mutant forms a complex with wild-type BchD. Mutant shows 10.9% of wild-type ATPase activity. Mutant is deficient of magnesium chelatase activity | Rhodobacter capsulatus |
V113G | BchI mutant forms a complex with wild-type BchD. Mutant shows 25% of wild-type ATPase activity. Mutant shows magnesium chelatase activity. Magnesium chelatase assays containing a mixture of mutant BchI V113G and wild-type BchI have the effect of stimulating the activity | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deuteroporphyrin IX + Mg2+ + H2O | - |
Rhodobacter capsulatus | ADP + phosphate + Mg-deuteroporphyrin IX + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BchID | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Rhodobacter capsulatus |