Literature summary for 6.5.1.9 extracted from

Matussek, K.; Moritz, P.; Brunner, N.; Eckerskorn, C.; Hensel, R.
Cloning, sequencing, and expression of the gene encoding cyclic 2,3-diphosphoglycerate synthetase, the key enzyme of cyclic 2,3-diphosphoglycerate metabolism in Methanothermus fervidus (1998), J. Bacteriol., 180, 5997-6004 .

Search for more literature for 6.5.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL1 Blue cells	Methanothermus fervidus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3	-	ATP	native enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
3	-	ATP	recombinant enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
4.1	-	ADP	recombinant enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
4.7	-	ADP	native enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
5.8	-	2,3-diphospho-D-glycerate	native enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
5.9	-	2,3-diphospho-D-glycerate	recombinant enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
41.2	-	cyclic 2,3-bisphosphoglycerate	native enzyme, at pH 7.0 and 83°C	Methanothermus fervidus
44.9	-	cyclic 2,3-bisphosphoglycerate	recombinant enzyme, at pH 7.0 and 83°C	Methanothermus fervidus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	875 mM used in assay conditions	Methanothermus fervidus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
112000	-	gel filtration	Methanothermus fervidus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + phosphate + cyclic 2,3-bisphosphoglycerate	Methanothermus fervidus	-	ATP + 2,3-diphospho-D-glycerate	-	r
ADP + phosphate + cyclic 2,3-bisphosphoglycerate	Methanothermus fervidus DSM 2088	-	ATP + 2,3-diphospho-D-glycerate	-	r
ATP + 2,3-diphospho-D-glycerate	Methanothermus fervidus	the enzyme shows a clear preference for the synthetic reaction	ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	r
ATP + 2,3-diphospho-D-glycerate	Methanothermus fervidus DSM 2088	the enzyme shows a clear preference for the synthetic reaction	ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	r

Organism

Organism	UniProt	Comment	Textmining
Methanothermus fervidus	O93732	-	-
Methanothermus fervidus DSM 2088	O93732	-	-

Purification (Commentary)

Purification (Comment)	Organism
phenyl-Sepharose column chromatography, hydroxyapatite column chromatography and Reactive Green 19 agarose column chromatography	Methanothermus fervidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.02	-	native enzyme from crude extract, at pH 7.0 and 83°C	Methanothermus fervidus
0.15	-	recombinant enzyme from crude extract, at pH 7.0 and 83°C	Methanothermus fervidus
15.6	-	recombinant enzyme after 104fold purification, at pH 7.0 and 83°C	Methanothermus fervidus
29	-	native enzyme after 1430fold purification, at pH 7.0 and 83°C	Methanothermus fervidus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	Methanothermus fervidus	ATP + 2,3-diphospho-D-glycerate	-	r
ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	Methanothermus fervidus DSM 2088	ATP + 2,3-diphospho-D-glycerate	-	r
ATP + 2,3-diphospho-D-glycerate	the enzyme shows a clear preference for the synthetic reaction	Methanothermus fervidus	ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	r
ATP + 2,3-diphospho-D-glycerate	the enzyme shows a clear preference for the synthetic reaction	Methanothermus fervidus DSM 2088	ADP + phosphate + cyclic 2,3-bisphosphoglycerate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 50657, calculated from amino acid sequence	Methanothermus fervidus
homodimer	2 * 57000-59000, SDS-PAGE	Methanothermus fervidus

Synonyms

Synonyms	Comment	Organism
cDPGs	-	Methanothermus fervidus
cpgS	-	Methanothermus fervidus
cyclic 2,3-diphosphoglycerate synthetase	-	Methanothermus fervidus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Methanothermus fervidus

General Information

General Information	Comment	Organism
metabolism	key enzyme of cyclic 2,3-diphosphoglycerate metabolism	Methanothermus fervidus

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Release 2023.1 (January 2023)