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Literature summary for 6.5.1.9 extracted from

  • Lehmacher, A.; Vogt, A.B.; Hensel, R.
    Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus (1990), FEBS Lett., 272, 94-98 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA the enzyme is almost completely inhibited by 5 mM EDTA Methanothermus fervidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
ATP at pH 7.0 and 75°C Methanothermus fervidus
5.5
-
2,3-diphospho-D-glycerate at pH 7.0 and 75°C Methanothermus fervidus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the maximally (2.4fold) activating KC1 concentration is 0.5 M Methanothermus fervidus
Mg2+ required for activity Methanothermus fervidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
gel filtration Methanothermus fervidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2,3-diphospho-D-glycerate Methanothermus fervidus
-
ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
?
ATP + 2,3-diphospho-D-glycerate Methanothermus fervidus DSM 2088
-
ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
?

Organism

Organism UniProt Comment Textmining
Methanothermus fervidus
-
-
-
Methanothermus fervidus DSM 2088
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, hydroxyapatite column chromatography, ATP-agarose column chromatography, and 2,3-diphospho-D-glycerate-agarose column chromatography Methanothermus fervidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
crude extract, at pH 7.0 and 75°C Methanothermus fervidus
20.6
-
after 1030fold purification, at pH 7.0 and 75°C Methanothermus fervidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2,3-diphospho-D-glycerate
-
Methanothermus fervidus ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
?
ATP + 2,3-diphospho-D-glycerate
-
Methanothermus fervidus DSM 2088 ADP + phosphate + cyclic 2,3-bisphosphoglycerate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 38000, SDS-PAGE Methanothermus fervidus

Synonyms

Synonyms Comment Organism
cDPGs
-
Methanothermus fervidus
cyclic 2,3-diphosphoglycerate synthetase
-
Methanothermus fervidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80
-
Methanothermus fervidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13
-
ATP at pH 7.0 and 75°C Methanothermus fervidus
13
-
2,3-diphospho-D-glycerate at pH 7.0 and 75°C Methanothermus fervidus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 the enzyme is active between pH 5.0 and 7.5 Methanothermus fervidus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Methanothermus fervidus