Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the enzyme is almost completely inhibited by 5 mM EDTA | Methanothermus fervidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
ATP | at pH 7.0 and 75°C | Methanothermus fervidus | |
5.5 | - |
2,3-diphospho-D-glycerate | at pH 7.0 and 75°C | Methanothermus fervidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | the maximally (2.4fold) activating KC1 concentration is 0.5 M | Methanothermus fervidus | |
Mg2+ | required for activity | Methanothermus fervidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
gel filtration | Methanothermus fervidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2,3-diphospho-D-glycerate | Methanothermus fervidus | - |
ADP + phosphate + cyclic 2,3-bisphosphoglycerate | - |
? | |
ATP + 2,3-diphospho-D-glycerate | Methanothermus fervidus DSM 2088 | - |
ADP + phosphate + cyclic 2,3-bisphosphoglycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermus fervidus | - |
- |
- |
Methanothermus fervidus DSM 2088 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, hydroxyapatite column chromatography, ATP-agarose column chromatography, and 2,3-diphospho-D-glycerate-agarose column chromatography | Methanothermus fervidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
crude extract, at pH 7.0 and 75°C | Methanothermus fervidus |
20.6 | - |
after 1030fold purification, at pH 7.0 and 75°C | Methanothermus fervidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2,3-diphospho-D-glycerate | - |
Methanothermus fervidus | ADP + phosphate + cyclic 2,3-bisphosphoglycerate | - |
? | |
ATP + 2,3-diphospho-D-glycerate | - |
Methanothermus fervidus DSM 2088 | ADP + phosphate + cyclic 2,3-bisphosphoglycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 38000, SDS-PAGE | Methanothermus fervidus |
Synonyms | Comment | Organism |
---|---|---|
cDPGs | - |
Methanothermus fervidus |
cyclic 2,3-diphosphoglycerate synthetase | - |
Methanothermus fervidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Methanothermus fervidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
ATP | at pH 7.0 and 75°C | Methanothermus fervidus | |
13 | - |
2,3-diphospho-D-glycerate | at pH 7.0 and 75°C | Methanothermus fervidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | the enzyme is active between pH 5.0 and 7.5 | Methanothermus fervidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanothermus fervidus |