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Literature summary for 6.5.1.6 extracted from

  • Kim, Y.J.; Lee, H.S.; Bae, S.S.; Jeon, J.H.; Yang, S.H.; Lim, J.K.; Kang, S.G.; Kwon, S.T.; Lee, J.H.
    Cloning, expression, and characterization of a DNA ligase from a hyperthermophilic archaeon Thermococcus sp. (2006), Biotechnol. Lett., 28, 401-407.
    View publication on PubMed
Search for more literature for 6.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermococcus onnurineus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, about 25% loss of activity Thermococcus onnurineus
Mn2+ 1 mM, about 50% loss of activity Thermococcus onnurineus
Ni2+ 1 mM, about 70% loss of activity Thermococcus onnurineus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM, 2.5fold stimulation Thermococcus onnurineus
Zn2+ 1 mM, 1.5 fold stimulation Thermococcus onnurineus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE Thermococcus onnurineus

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus Q2Q452
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus onnurineus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m about 10% of the activity compared to ATP Thermococcus onnurineus AMP + phosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme displays nick joining and blunt-end ligation activity. ATP and NAD+ are comparably active Thermococcus onnurineus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
GTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m about 20% of the activity compared to ATP Thermococcus onnurineus GMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme displays nick joining and blunt-end ligation activity. ATP and NAD+ are comparably active Thermococcus onnurineus AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?
TTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m about 20% of the activity compared to ATP Thermococcus onnurineus TMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
UTP + (deoxyribonucleotide)n + (deoxyribonucleotide)m about 15% of the activity compared to ATP Thermococcus onnurineus UMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Thermococcus onnurineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermococcus onnurineus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 90 65°C: about 50% of maximal activity, 90°C: about 95% of maximal activity Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermococcus onnurineus
8
-
 assay at Thermococcus onnurineus

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity Thermococcus onnurineus