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Literature summary for 6.5.1.6 extracted from

  • Rolland, J.L.; Gueguen, Y.; Persillon, C.; Masson, J.M.; Dietrich, J.
    Characterization of a thermophilic DNA ligase from the archaeon Thermococcus fumicolans (2004), FEMS Microbiol. Lett., 236, 267-273.
    View publication on PubMed
Search for more literature for 6.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS Thermococcus fumicolans

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ while optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 Thermococcus fumicolans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 NAD+ pH 6.5, 55°C Thermococcus fumicolans
1.16
-
 ATP pH 6.5, 55°C Thermococcus fumicolans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl Thermococcus fumicolans
Mg2+ strongly dependent on the MgCl2. While optimal activity is obtained with 2 mM, it is almost totally inhibited with 10 mM MgCl2 Thermococcus fumicolans
Na+ the enzyme does not require monovalent cations to be active. 2 mM KCl or up to 5 mM of NaCl increase its activity up to 70%. Higher concentrations of these cations are inhibitory, but the enzyme still retains 50% activity with 100 mM NaCl or 100 mM KCl Thermococcus fumicolans

Organism

Organism UniProt Comment Textmining
Thermococcus fumicolans Q9HH07
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus fumicolans

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide (2) Thermococcus fumicolans
a
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate (1) Thermococcus fumicolans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor Thermococcus fumicolans AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m the enzyme displays nick joining and blunt-end ligation activity using either ATP or NAD+, as a cofactor Thermococcus fumicolans AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
Tfu DNA ligase
-
 Thermococcus fumicolans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermococcus fumicolans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: 50% of maximal activity, 80°C: about 50% of maximal activity Thermococcus fumicolans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 3 h, stable Thermococcus fumicolans
90
-
 15 min, 50% loss of activity Thermococcus fumicolans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermococcus fumicolans

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 about 80% of maximal activity, pH 7.0: optimum, pH 7.5: about 10% of maximal activity Thermococcus fumicolans