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Literature summary for 6.5.1.5 extracted from

  • Sato, A.; Soga, T.; Igarashi, K.; Takesue, K.; Tomita, M.; Kanai, A.
    GTP-dependent RNA 3'-terminal phosphate cyclase from the hyperthermophilic archaeon Pyrococcus furiosus (2011), Genes Cells, 16, 1190-1199.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the recombinant C-terminal His-tagged protein in the Escherichia coli strain BL21(DE3)pLysS Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
GMP
-
Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation, Mn2+ can substitute for Mg2+ Pyrococcus furiosus
Mn2+ activation, Mn2+ can substitute for Mg2+ Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + RNA 3'-terminal-phosphate Pyrococcus furiosus the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U0N7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + RNA 3'-terminal-phosphate the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase Pyrococcus furiosus GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?
GTP + RNA 3'-terminal-phosphate the enzyme utilizes GTP approximately 10times more effectively than ATP. UTP and CTP have no effect on activity. Oligoribonucleotide substrates of different lengths (4-, 8- and 12-mers) are tested, the 4-mer is the most efficient substrate Pyrococcus furiosus GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
Pf-Rtc
-
Pyrococcus furiosus
PF1549
-
Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 95 similar activity in the range 22°C-75°C, much higher activity at 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase Pyrococcus furiosus