Cloned (Comment) | Organism |
---|---|
overexpression of the recombinant C-terminal His-tagged protein in the Escherichia coli strain BL21(DE3)pLysS | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GMP | - |
Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation, Mn2+ can substitute for Mg2+ | Pyrococcus furiosus | |
Mn2+ | activation, Mn2+ can substitute for Mg2+ | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNA 3'-terminal-phosphate | Pyrococcus furiosus | the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U0N7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNA 3'-terminal-phosphate | the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase | Pyrococcus furiosus | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
GTP + RNA 3'-terminal-phosphate | the enzyme utilizes GTP approximately 10times more effectively than ATP. UTP and CTP have no effect on activity. Oligoribonucleotide substrates of different lengths (4-, 8- and 12-mers) are tested, the 4-mer is the most efficient substrate | Pyrococcus furiosus | GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
Pf-Rtc | - |
Pyrococcus furiosus |
PF1549 | - |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 95 | similar activity in the range 22°C-75°C, much higher activity at 95°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus furiosus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme may contribute to GTP-dependent RNA ligation activity through the 2'-5' RNA ligase | Pyrococcus furiosus |