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Literature summary for 6.5.1.3 extracted from

  • Unciuleac, M.C.; Goldgur, Y.; Shuman, S.
    Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase (2015), Proc. Natl. Acad. Sci. USA, 112, 13868-13873 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M HEPES pH 6.5, 30% (w/v) PEG6000 Naegleria gruberi

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the enzyme prefers manganese as the metal cofactor for nick sealing Naegleria gruberi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m Naegleria gruberi
-
 (ribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [RNA ligase]-L-lysine Naegleria gruberi
-
 [RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Naegleria gruberi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
-
 Naegleria gruberi (ribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [RNA ligase]-L-lysine
-
 Naegleria gruberi [RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP-dependent RNA ligase
-
 Naegleria gruberi
RNL
-
 Naegleria gruberi