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Literature summary for 6.5.1.3 extracted from

  • Desai, K.K.; Cheng, C.L.; Bingman, C.A.; Phillips, G.N.; Raines, R.T.
    A tRNA splicing operon Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation (2014), Nucleic Acids Res., 42, 3931-3942 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
archease
-
 Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
D65A inactive Pyrococcus horikoshii
D95A the mutant shows RNA 3'-P guanylylation activity Pyrococcus horikoshii
E206A the mutant shows strongly reduced activity compared to the wild type enzyme Pyrococcus horikoshii
F204A inactive Pyrococcus horikoshii
H203A inactive Pyrococcus horikoshii
H404A inactive Pyrococcus horikoshii
K480A the mutant shows 2fold increased activity compared to the wild type enzyme Pyrococcus horikoshii
N202A inactive Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m Pyrococcus horikoshii
-
 (ribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [RNA ligase]-L-lysine Pyrococcus horikoshii
-
 [RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
-
 Pyrococcus horikoshii (ribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [RNA ligase]-L-lysine
-
 Pyrococcus horikoshii [RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
rtcB
-
 Pyrococcus horikoshii