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Literature summary for 6.5.1.3 extracted from

  • Zhelkovsky, A.M.; McReynolds, L.A.
    Simple and efficient synthesis of 5' pre-adenylated DNA using thermostable RNA ligase (2011), Nucleic Acids Res., 39, e117.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis efficient synthesis of 5' pre-adenylated DNA Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes an adenylation reaction that is specific for ATP and either ssDNA or RNA. It does not require synthesis of a template strand for adenylation. To achieve quantitative DNA adenylation, single turnover reaction condition with substrate to enzyme ratio 1:1 should be used Methanothermobacter thermautotrophicus ?
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
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Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 65 optimal for stability Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 in the presence of Mg2+, the reaction has a pH optimum of 6.0–6.5 Methanothermobacter thermautotrophicus