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Literature summary for 6.5.1.3 extracted from

  • Nelissen, F.H.; van Gammeren, A.J.; Tessari, M.; Girard, F.C.; Heus, H.A.; Wijmenga, S.S.
    Multiple segmental and selective isotope labeling of large RNA for NMR structural studies (2008), Nucleic Acids Res., 36, e89.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis selective isotope labeling of RNA by introducing a labeled RNA segment via a two-step protocol, which uses T4 DNA ligase and T4 RNA ligase 1, and a one-pot protocol, which uses T4 RNA ligase 1 alone. Protection of termini is not required, provided segmentation sites can be chosen such that the segments fold into the target structure or target-like structures and thus are not trapped into stable alternate structures. Labeling protocols are generally applicable to large RNA molecules and can be extended to more than three segments Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
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