Application | Comment | Organism |
---|---|---|
molecular biology | RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) | Phage TS2126 |
molecular biology | the properties of TS2126 RNA ligase 1 makes it very attractive for processes like adaptor ligation, and single-stranded solid phase gene synthesis | Phage TS2126 |
Cloned (Comment) | Organism |
---|---|
RNA ligase gene is inserted into a pET23b expression vector with a C-terminal His6-tag. This vector is transformed into Escherichia coli strain BL21 | Phage TS2126 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | above 2.5 mM, complete inhibition at 10 mM. The inhibition can be partially rescued by adding more Mg2+ to the reaction | Phage TS2126 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
ATP | activity with RNA | Phage TS2126 | |
0.04 | - |
ATP | activity with DNA | Phage TS2126 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, optimum: 5-10 mM | Phage TS2126 | |
Mn2+ | activates, showing slightly higher activity than Mg2+ | Phage TS2126 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phage TS2126 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phage TS2126 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | the TS2126 RNA ligase catalyzes both inter- and intra-molecular single-stranded DNA ligation to more than 50% completion in not more than a few hours at an elevated temperature, although favoring intramolecular ligation on RNA and single-stranded DNA substrates | Phage TS2126 | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA ligase 1 | - |
Phage TS2126 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Phage TS2126 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 35% of maximal activity, 75°C: about 60% of maximal activity | Phage TS2126 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
realatively stable at | Phage TS2126 |
80 | - |
loses all activity above | Phage TS2126 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | MOPS buffer | Phage TS2126 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 40% of maximal activity, pH 8.5: about 30% of maximal activity | Phage TS2126 |