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Literature summary for 6.5.1.3 extracted from

  • Deng, J.; Schnaufer, A.; Salavati, R.; Stuart, K.D.; Hol, W.G.
    High resolution crystal structure of a key editosome enzyme from Trypanosoma brucei:RNA editing ligase 1 (2004), J. Mol. Biol., 343, 601-613.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21goldDE3 Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the catalytic domain of TbREL1 at 1.2 A resolution, in complex with ATP and magnesium.The magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms. Sitting-drop, vapor-diffusion with 100 mM HEPES, 100 mM Mg2+ and 35% (w/v) PEG3350 at pH 7.0. The crystal belongs to space group P2(1), with cell dimensions of a = 44.9 A, b = 58.6 A, c = 53.0 A and beta = 100.2°, and one molecule in the asymmetric unit Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Trypanosoma brucei the enzyme is essential for survival of both insect and bloodstream forms of the parasite AMP + diphosphate + (ribonucleotide)n+m
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?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
Trypanosoma brucei AMP + diphosphate + (ribonucleotide)n+m
-
?
ATP + (ribonucleotide)n + (ribonucleotide)m the enzyme is essential for survival of both insect and bloodstream forms of the parasite Trypanosoma brucei AMP + diphosphate + (ribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
RNA editing ligase 1
-
Trypanosoma brucei
TbREL1
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Trypanosoma brucei