Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21goldDE3 | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the catalytic domain of TbREL1 at 1.2 A resolution, in complex with ATP and magnesium.The magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms. Sitting-drop, vapor-diffusion with 100 mM HEPES, 100 mM Mg2+ and 35% (w/v) PEG3350 at pH 7.0. The crystal belongs to space group P2(1), with cell dimensions of a = 44.9 A, b = 58.6 A, c = 53.0 A and beta = 100.2°, and one molecule in the asymmetric unit | Trypanosoma brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | magnesium ion interacts with the beta and gamma-phosphate groups and is almost perfectly octahedrally coordinated by six phosphate and water oxygen atoms | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | Trypanosoma brucei | the enzyme is essential for survival of both insect and bloodstream forms of the parasite | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | - |
Trypanosoma brucei | AMP + diphosphate + (ribonucleotide)n+m | - |
? | |
ATP + (ribonucleotide)n + (ribonucleotide)m | the enzyme is essential for survival of both insect and bloodstream forms of the parasite | Trypanosoma brucei | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA editing ligase 1 | - |
Trypanosoma brucei |
TbREL1 | - |
Trypanosoma brucei |