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Literature summary for 6.5.1.3 extracted from

  • Ho, C.K.; Shuman, S.
    Bacteriophage T4 RNA ligase 2 (gp24.1) exemplifies a family of RNA ligases found in all phylogenetic domains (2002), Proc. Natl. Acad. Sci. USA, 99, 12709-12714.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
transformed into Escherichia coli BL21(DE3) Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
E204A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
H37A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
H37D substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
K225A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
K227A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4
K35A substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 recombinant protein, adsorption to Ni-agarose and step-elution with imidazole Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4 participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (ribonucleotide)n + (ribonucleotide)m participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
gp24.1
-
 Tequatrovirus T4
Rnl2
-
 Tequatrovirus T4
T4 RNA ligase 2
-
 Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7
-
 Tequatrovirus T4
6.5
-
 adenylyltransferase activity Tequatrovirus T4

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 adenylyltransferase activity Tequatrovirus T4