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Literature summary for 6.5.1.3 extracted from

  • Yin, S.; Ho, C.K.; Shuman, S.
    Structure-function analysis of T4 RNA ligase 2 (2003), J. Biol. Chem., 278, 17601-17608.
    View publication on PubMed
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
encoded by T4 gene 24.1, mutants expressed in Escherichia coli Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
D120A alanine-scanning mutagenesis Tequatrovirus T4
E34A alanine-scanning mutagenesis Tequatrovirus T4
E34D substitution mutant, introduced into the rnl2 gene by PCR Tequatrovirus T4
E34Q substitution mutant, introduced into the rnl2 gene by PCR Tequatrovirus T4
E99A alanine-scanning mutagenesis Tequatrovirus T4
F119A alanine-scanning mutagenesis Tequatrovirus T4
K189A alanine-scanning mutagenesis Tequatrovirus T4
K209A alanine-scanning mutagenesis Tequatrovirus T4
N40A alanine-scanning mutagenesis Tequatrovirus T4
N40D substitution mutant, introduced into the rnl2 gene by PCR Tequatrovirus T4
N40Q substitution mutant, introduced into the rnl2 gene by PCR Tequatrovirus T4
N40R substitution mutant, introduced into the rnl2 gene by PCR Tequatrovirus T4
R55A alanine-scanning mutagenesis Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4 RNA-editing AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzymes Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (ribonucleotide)n + (ribonucleotide)m RNA-editing Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
Rnl2
-
 Tequatrovirus T4
T4 RNA ligase 2
-
 Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Tequatrovirus T4