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Literature summary for 6.5.1.3 extracted from

  • Bergman, N.H.; Johnston, W.K.; Bartel, D.P.
    Kinetic framework for ligation by an efficient RNA ligase ribozyme (2000), Biochemistry, 39, 3115-3123.
    View publication on PubMed
Search for more literature for 6.5.1.3

Organism

Organism UniProt Comment Textmining
synthetic construct
-
 isolated from random sequences, pool of random sequences based on their ability to join a substrate oligonucleotide to their own 5'-terminus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of diphosphate, reverse of ligation has been detected, but very inefficient, not saturable by soluble amounts of diphosphate synthetic construct AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (RNA)n
-
 synthetic construct ?
-
 ?
ATP + RNA + 5'-aaCCAGUC DNA-RNA hybrid synthetic construct AMP + diphosphate + ?
-
 ?

Synonyms

Synonyms Comment Organism
class I RNA ligase ribozyme
-
 synthetic construct

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
 (RNA)n pH 6.0, 22°C, multiple-turnover derivatives 210t and 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
0.266
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
0.583
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
2.33
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
2.94
-
 (RNA)n pH 8.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 210t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct
6.08
-
 (RNA)n pH 9.0, 22°C, multiple-turnover derivative 207t, engineered by changing the arrangement of the base pairs in the P2 stem synthetic construct