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Literature summary for 6.5.1.3 extracted from

  • Last, J.A.; Anderson, W.F.
    Purification and properties of bacteriophage T4-induced RNA ligase (1976), Arch. Biochem. Biophys., 174, 167-176.
    View publication on PubMed
Search for more literature for 6.5.1.3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 45000, SDS-PAGE after reduction with 2-mercaptoethanol Tequatrovirus T4
47000 48200 gel filtration, high-speed sedimentation equilibrium centrifugation Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Storage Stability

Storage Stability Organism
in liquid nitrogen, at 0.5 mg protein per ml, stable for at least 6 months Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m formation of a phosphodiester bond between a 5'-PO4-terminated oligonucleotide and a 3'-OH terminated oligonucleotide. Intramolecular formation of single-stranded circles with longer oligonucleotides of the type pAp(Ap)nAOH, where n is about 15 or greater. Intermolecular joining of pAp(Ap)3AOH, where the 5'-PO4-terminated oligonucleotide is short enough to prevent apposition of its 3' and 5' ends, to UpUpUOH Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE after reduction with 2-mercaptoethanol Tequatrovirus T4