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Literature summary for 6.5.1.2 extracted from
- Structure of the adenylation domain of NAD+-dependent DNA ligase from Staphylococcus aureus (2009), Acta Crystallogr. Sect. F, 65, 1078-1082.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 2.1-2.5 M ammonium sulfate and 0.1 M MES or HEPES pH 6.8-7.5 | Staphylococcus aureus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75080 | - |
calculated from amino acid sequence | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | Q9AIU7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap FF column chromatography, hydroxyapatite type I column chromatography, and HiPrep Q column chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Staphylococcus aureus | AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LigA | - |
Staphylococcus aureus |
| NAD+-dependent DNA ligase | - |
Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Staphylococcus aureus | |
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Release 2023.1 (January 2023)
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