D450A |
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks |
Escherichia coli |
D452A |
mutant with 68% of wild type activity |
Escherichia coli |
D551A |
mutant with 120% of wild type activity |
Escherichia coli |
D551A |
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks |
Escherichia coli |
E519A |
mutant with 71% of wild type activity |
Escherichia coli |
E519A |
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks |
Escherichia coli |
G489A |
the mutant retains 25% of wild type activity |
Escherichia coli |
G489D |
mutant with 0.8% of wild type activity |
Escherichia coli |
G489V |
mutant with 3.2% of wild type activity |
Escherichia coli |
G521A |
the mutant is 50fold less active than wild type LigA |
Escherichia coli |
G521D |
mutant with less than 0.1% of wild type function |
Escherichia coli |
G521V |
mutant with less than 0.1% of wild type function |
Escherichia coli |
G553A |
mutant with 38% of wild type activity |
Escherichia coli |
G553D |
mutant with 2.6% of wild type activity |
Escherichia coli |
G553V |
mutant with 0.9% of wild type activity |
Escherichia coli |
I384A |
the mutant is defective for nick sealing, retaining 4% of wild type activity |
Escherichia coli |
K627A |
mutant with 40% of wild type activity |
Escherichia coli |
K634A/K635A |
mutant with 44% of wild type activity |
Escherichia coli |
K648A |
mutant with 37% of wild type activity |
Escherichia coli |
K651A |
mutant with 35% of wild type activity |
Escherichia coli |
N355A |
mutant with 30% of wild type activity |
Escherichia coli |
Q330A |
the mutation has no apparent effect on nick sealing acivity |
Escherichia coli |
Q386A |
mutant with 30% of wild type activity |
Escherichia coli |
R333A |
the oligonucleotide-binding domain mutation strongly suppresses DNA relaxation |
Escherichia coli |
R379A |
the oligonucleotide-binding domain mutation strongly suppresses DNA relaxation |
Escherichia coli |
R487A |
mutant with 6% of wild type activity |
Escherichia coli |
R510A |
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks |
Escherichia coli |
R614A |
mutant with 14% of wild type activity |
Escherichia coli |
V383A |
the mutant is defective for nick sealing, retaining 4% of wild type activity |
Escherichia coli |