Any feedback?
Literature summary for 6.5.1.2 extracted from
- Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans (2007), BMC Mol. Biol., 8, 69.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K128A | mutant enzyme shows no ligation activity | Deinococcus radiodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | 1 mM | Deinococcus radiodurans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.105 | - |
nicked DNA | - |
Deinococcus radiodurans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10fold lower activation than Mn2+, maximal activity at 2 mM | Deinococcus radiodurans | |
| Mg2+ | 10fold lower activation than Mn2+, maximal activity at 1 mM | Deinococcus radiodurans | |
| Mn2+ | preference for Mn2+ as cofactor, maximal activity at 1 mM | Deinococcus radiodurans | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicked DNA | - |
Deinococcus radiodurans | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Deinococcus radiodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Deinococcus radiodurans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
