Protein Variants | Comment | Organism |
---|---|---|
A644I | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
E654A | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
G617I | mutation in BRCT domain, mutant enzyme does not shows any detectable ligation with nicked substrate in excess | Thermus sp. AK16D |
G634I | mutation in BRCT domain, ligation activity is about 15% of the activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
G638I | mutation in BRCT domain, ligation activity is 30-40% lower than that with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
K619R | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
K640R | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
L647A | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
R606A | mutation in BRCT domain, ligation activity is 30-40% lower than that with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
S623A | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
T599A | mutation in BRCT domain, ligation activity is 30-40% lower than that with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
V624A | mutation in BRCT domain, comparable ligation activity with wild-type enzyme with nicked substrate in excess | Thermus sp. AK16D |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. AK16D | - |
- |
- |