Application | Comment | Organism |
---|---|---|
medicine | potential target for antibiotics due to the fact that eukaryotic cells use ATP instead of NAD+ as cofactor for DNA-ligase | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
1-318 fragment with K114A mutation | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour phase diffusion method, K114A mutant protein fragment | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
K114A | prevention of partial adenylation of the enzyme | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Geobacillus stearothermophilus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Geobacillus stearothermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Geobacillus stearothermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Geobacillus stearothermophilus |