BRENDA - Enzyme Database show
show all sequences of 6.5.1.2

Cofactor Binding modulates the conformational stabilities and unfolding patterns of NAD+-dependent DNA ligases from Escherichia coli and Thermus scotoductus

Georlette, D.; Blaise, V.; Dohmen, C.; Bouillenne, F.; Damien, B.; Depiereux, E.; Gerday, C.; Uversky, V.N.; Feller, G.; J. Biol. Chem. 278, 49945-49953 (2003)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
potential target for antibiotics
Escherichia coli
medicine
potential target for antibiotics
Thermus scotoductus
Cloned(Commentary)
Commentary
Organism
-
Thermus scotoductus
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
Escherichia coli
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
Thermus scotoductus
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
Thermus scotoductus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Thermus scotoductus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Thermus scotoductus
-
Escherichia coli
Reaction
Reaction
Commentary
Organism
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond
Thermus scotoductus
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
652489
Escherichia coli
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
652489
Thermus scotoductus
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Thermus scotoductus
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
652489
Escherichia coli
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
652489
Thermus scotoductus
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Thermus scotoductus
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
thermal stability is increased by cofactor binding
Escherichia coli
additional information
-
thermal stability is increased by cofactor binding
Thermus scotoductus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required, binding of cofactor induces conformational rearangement within the active site
Escherichia coli
NAD+
required, binding of cofactor induces conformational rearangement within the active site
Thermus scotoductus
Application (protein specific)
Application
Commentary
Organism
medicine
potential target for antibiotics
Escherichia coli
medicine
potential target for antibiotics
Thermus scotoductus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Thermus scotoductus
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required, binding of cofactor induces conformational rearangement within the active site
Escherichia coli
NAD+
required, binding of cofactor induces conformational rearangement within the active site
Thermus scotoductus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
Escherichia coli
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
Thermus scotoductus
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
Thermus scotoductus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermus scotoductus
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
652489
Escherichia coli
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
652489
Thermus scotoductus
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Thermus scotoductus
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
652489
Escherichia coli
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Escherichia coli
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
652489
Thermus scotoductus
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
652489
Thermus scotoductus
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
thermal stability is increased by cofactor binding
Escherichia coli
additional information
-
thermal stability is increased by cofactor binding
Thermus scotoductus
Other publictions for EC 6.5.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748720
Korycka-Machala
Naphthalimides selectively in ...
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Molecules
22
pii: E154
2017
-
-
-
-
-
-
3
-
-
-
-
2
-
160
-
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1
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2
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1
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1
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1
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2
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-
748787
Konomura
Rad51 and RecA juxtapose dsDN ...
Escherichia coli
Nucleic Acids Res.
45
337-352
2017
2
-
-
-
-
-
-
-
-
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-
1
-
1
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1
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1
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1
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747327
Pergolizzi
Biochemical and structural ch ...
Escherichia coli, Haemophilus influenzae, Mycobacterium tuberculosis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae
Biosci. Rep.
36
e00391
2016
-
-
-
-
-
-
129
-
-
-
-
6
-
6
-
-
-
-
-
-
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-
6
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6
-
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64
-
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6
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64
129
-
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6
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6
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748783
Chauleau
Kinetic mechanism and fidelit ...
Escherichia coli
Nucleic Acids Res.
44
2298-2309
2016
-
-
1
-
-
-
-
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-
1
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1
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2
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1
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2
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1
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1
1
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1
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1
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1
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2
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-
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-
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1
1
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-
746763
Shrivastava
Homology modeling of NAD+-dep ...
Wolbachia sp. subsp. Brugia malayi, Wolbachia sp. subsp. Brugia malayi TRS
Antimicrob. Agents Chemother.
59
3736-3747
2015
-
-
1
-
-
-
7
-
-
-
-
2
-
11
-
-
1
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-
-
-
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2
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1
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7
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1
1
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7
7
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2
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1
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2
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-
747273
Murphy-Benenato
Negishi cross-coupling enable ...
Escherichia coli, Escherichia coli ARC524, Haemophilus influenzae, Haemophilus influenzae ARM158, Staphylococcus aureus, Staphylococcus aureus ARC561, Streptococcus pneumoniae, Streptococcus pneumoniae ARC548
Bioorg. Med. Chem. Lett.
25
5172-5177
2015
-
-
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168
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8
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8
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8
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4
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124
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4
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124
168
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8
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8
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747798
Giroux
Inhibiting NAD+-dependent DNA ...
Haloferax volcanii
FEMS Microbiol. Lett.
362
pii: fnv181
2015
-
-
-
-
-
-
1
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-
-
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1
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3
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1
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1
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1
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748806
Yadav
Tricyclic dihydrobenzoxazepin ...
Mycobacterium tuberculosis
Org. Biomol. Chem.
13
5475-5487
2015
-
-
-
-
-
-
3
-
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1
-
5
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1
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1
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748807
Pergolizzi
Base-modified NAD and AMP der ...
Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Org. Biomol. Chem.
13
6380-6398
2015
-
-
-
-
-
-
8
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3
-
161
-
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3
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8
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3
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749394
Hale
-
From fragments to leads Novel ...
Haemophilus influenzae, Staphylococcus aureus, Staphylococcus aureus ATCC 29213, Streptococcus pneumoniae, Streptococcus pneumoniae ATCC 10813
Tetrahedron Lett.
56
3108-3112
2015
-
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30
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5
-
8
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5
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30
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5
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5
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727155
Murphy-Benenato
Identification through structu ...
Enterococcus faecalis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
24
360-366
2014
-
-
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64
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5
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63
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746739
Jiang
A simple and highly sensitive ...
Escherichia coli
Anal. Chim. Acta
844
70-74
2014
-
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1
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1
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747433
Shapiro
Complete steady-state rate eq ...
Haemophilus influenzae
BMC Res. Notes
7
287
2014
-
-
-
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2
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1
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1
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747637
Sorci
NAD homeostasis in the bacter ...
Desulfovibrio alaskensis, Desulfovibrio vulgaris, Gluconobacter oxydans, Lawsonia intracellularis, Pelobacter carbinolicus, Synechococcus elongatus, Thermotoga maritima
DNA Repair
23
17-26
2014
-
-
-
-
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-
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7
-
7
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7
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726920
Jahic
The kinetic mechanism of S. pn ...
Streptococcus pneumoniae
Biochem. Pharmacol.
84
654-660
2012
-
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1
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4
3
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1
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4
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727142
Wang
Design, synthesis and biologic ...
Escherichia coli
Bioorg. Med. Chem. Lett.
22
3699-3703
2012
-
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32
-
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32
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32
32
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727145
Surivet
Structure-guided design, synth ...
Escherichia coli
Bioorg. Med. Chem. Lett.
22
6705-6711
2012
-
-
-
-
-
-
31
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
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31
-
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31
31
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728580
Shrivastava
Molecular characterization of ...
Wolbachia endosymbiont of Brugia malayi
PLoS ONE
7
e41113
2012
-
-
1
-
-
-
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2
-
6
1
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4
-
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1
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2
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1
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1
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1
1
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1
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2
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6
1
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1
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2
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1
-
1
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1
1
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1
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1
-
-
713771
Mills
Novel bacterial NAD+-dependent ...
Escherichia coli, Escherichia coli MG1655, Haemophilus influenzae, Haemophilus influenzae KW20, Mycoplasma pneumoniae, Mycoplasma pneumoniae M29, Staphylococcus aureus, Staphylococcus aureus RN4220, Streptococcus pneumoniae
Antimicrob. Agents Chemother.
55
1088-1096
2011
-
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25
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5
5
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25
25
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5
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9
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A high-throughput fluorescence ...
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1
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Virtual screening of specific ...
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Electrochemical detection of n ...
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2
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1
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Discovery of bacterial NAD+-de ...
Staphylococcus aureus, Streptococcus pneumoniae
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2
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30
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Luan
Hairpin DNA probe based surfac ...
Escherichia coli
Analyst
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414-418
2010
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1
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Doi
Comparison of the molecular in ...
Escherichia virus T4, Thermus aquaticus
J. Biochem.
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697-703
2010
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2
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6
-
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710492
Singh
Toward the virtual screening o ...
Mycobacterium tuberculosis
Protein Pept. Lett.
17
269-276
2010
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4
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1
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4
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714854
Le
-
Properties of an NAD+-dependen ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Enzyme Microb. Technol.
46
113-117
2010
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1
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3
1
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4
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1
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4
1
1
1
1
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1
1
1
2
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1
2
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3
1
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1
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-
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4
1
1
1
1
-
1
1
1
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-
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-
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706961
Han
Structure of the adenylation d ...
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Acta Crystallogr. Sect. F
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1078-1082
2009
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1
1
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-
-
-
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1
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3
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1
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1
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1
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1
1
1
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1
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1
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1
-
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-
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707538
Ignatov
DNA ligases from thermophilic ...
Thermus aquaticus, Thermus thermophilus
Biochemistry
74
557-561
2009
-
-
2
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-
-
-
-
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2
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2
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2
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2
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2
2
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2
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2
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2
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-
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-
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2
2
-
-
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707630
Yutin
Evolution of DNA ligases of nu ...
Acanthamoeba polyphaga Mimivirus, Acidithiobacillus ferrooxidans ATCC 23270, Aedes taeniorhynchus iridescent virus, Amsacta moorei entomopoxvirus, Aquifex aeolicus VF5, Bacillus thuringiensis Bt407, Borrelia hermsii HS1, Brachyspira hyodysenteriae, Candidatus Blochmannia floridanus, Clostridium carboxidivorans P7, Desulfatibacillum alkenivorans AK-01, Desulfuromonas acetoxidans DSM 684, Dictyostelium discoideum AX4, Haloquadratum walsbyi DSM 16790, Invertebrate iridescent virus 6, Lentisphaera araneosa HTCC2155, Leptotrichia buccalis C-1013-b, Marinobacter sp. ELB17, Melanoplus sanguinipes entomopoxvirus, Methylacidiphilum infernorum V4, Natronomonas pharaonis DSM 2160, Nitratiruptor sp. SB155-2, Ostreococcus lucimarinus CCE9901, Plesiocystis pacifica SIR-1, Ralstonia phage phiRSL1, Rickettsia felis URRWXCal2, Sorangium cellulosum
Biol. Direct
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2009
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27
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27
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27
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27
-
-
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27
-
-
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27
-
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-
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27
-
-
-
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-
-
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-
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709023
Shuman
DNA ligases: progress and pros ...
Enterococcus faecalis, Escherichia coli
J. Biol. Chem.
284
17365-17369
2009
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-
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1
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2
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2
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2
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1
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2
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1
1
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2
-
-
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-
-
-
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-
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-
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690421
Vacek
Label-free electrochemical mon ...
Escherichia coli
Anal. Chem.
80
7609-7613
2008
-
-
-
-
-
-
-
-
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-
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
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690424
Li
Simultaneous identification of ...
Escherichia coli
Analyst
133
939-945
2008
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-
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1
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1
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-
-
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1
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1
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-
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1
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-
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1
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-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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691966
Pascal
DNA and RNA ligases: structura ...
Enterococcus faecalis, Escherichia coli, Thermus filiformis
Curr. Opin. Struct. Biol.
18
96-105
2008
-
-
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3
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-
-
-
-
-
-
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3
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-
-
-
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-
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3
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-
-
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-
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3
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
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-
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692315
Meier
Identification and characteriz ...
Streptococcus pneumoniae
FEBS J.
275
5258-5271
2008
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1
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-
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3
1
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2
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2
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1
-
-
-
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1
1
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-
-
1
-
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-
1
1
-
-
-
-
1
1
-
-
-
-
3
1
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
1
-
-
-
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-
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-
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693115
Wang
Structure-guided mutational an ...
Escherichia coli
J. Biol. Chem.
283
23343-23352
2008
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1
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27
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1
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1
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1
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1
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-
-
-
-
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1
-
-
-
-
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1
1
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27
-
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-
-
-
-
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1
-
-
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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693979
Dube
-
NAD+-dependent DNA ligase (Rv3 ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Med. Chem. Res.
17
189-198
2008
-
-
-
-
-
-
5
-
-
-
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5
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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5
-
-
-
1
-
-
-
5
5
-
-
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-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693984
Dwivedi
NAD+-dependent DNA ligase: a n ...
Enterococcus faecalis, Escherichia coli, Geobacillus stearothermophilus, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Thermus filiformis
Med. Res. Rev.
28
545-568
2008
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5
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21
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12
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-
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6
-
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5
-
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17
-
5
-
5
-
-
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17
21
-
-
-
-
-
-
-
-
-
-
-
-
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6
-
-
-
-
-
-
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-
-
-
-
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694991
Le
Biochemical characterization o ...
Deinococcus radiodurans
Protein Pept. Lett.
15
600-605
2008
-
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1
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-
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1
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4
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1
-
-
-
-
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1
-
1
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
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1
-
-
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
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671030
Feng
Mutational analysis of bacteri ...
Thermus sp., Thermus sp. TAK16D
Acta Biochim. Biophys. Sin.
39
608-616
2007
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-
-
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4
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-
-
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2
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3
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2
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4
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2
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
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-
-
-
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671312
Miesel
A high-throughput assay for th ...
Escherichia coli
Anal. Biochem.
366
9-17
2007
-
1
1
-
-
-
8
-
-
-
-
-
-
1
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-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
1
-
-
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4
8
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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671380
Korycka-Machala
Evaluation of NAD+-dependent D ...
Mycolicibacterium smegmatis
Antimicrob. Agents Chemother.
51
2888-2897
2007
-
1
-
-
-
-
-
-
-
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1
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6
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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672811
Blasius
Enzymes involved in DNA ligati ...
Deinococcus radiodurans
BMC Mol. Biol.
8
69
2007
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-
-
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1
-
1
1
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3
-
-
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1
-
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1
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-
-
-
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-
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1
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-
-
1
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-
-
-
-
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-
-
-
-
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1
-
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1
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
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675929
Nandakumar
Last stop on the road to repai ...
Escherichia coli
Mol. Cell
26
257-271
2007
-
-
-
1
-
-
-
-
-
-
-
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1
-
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1
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-
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-
-
-
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-
-
-
-
-
-
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-
-
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1
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-
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1
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-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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677062
Srivastava
NAD+-dependent DNA ligase (Rv3 ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proteins
69
97-111
2007
-
-
-
-
1
-
2
1
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2
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10
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1
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1
1
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2
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1
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2
2
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1
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2
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-
-
-
-
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1
-
-
-
-
-
1
1
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672808
Poidevin
Biochemical characterisation o ...
Haloferax volcanii
BMC Mol. Biol.
7
44
2006
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1
1
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2
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1
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6
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1
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-
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1
1
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2
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
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673070
Tomkinson
DNA ligases: Structure, reacti ...
Enterococcus faecalis, Thermus filiformis
Chem. Rev.
106
687-699
2006
-
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2
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-
-
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2
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-
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676060
Zhao
ATP- and NAD+-dependent DNA li ...
Haloferax volcanii
Mol. Microbiol.
59
743-752
2006
-
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-
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-
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7
-
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1
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677247
Benarroch
Characterization of mimivirus ...
Mimivirus
Virology
353
133-143
2006
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1
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6
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1
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5
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6
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1
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1
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-
660708
Zauner
Tethered DNA hairpins facilita ...
Escherichia coli
Analyst
130
345-349
2005
-
-
-
-
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1
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-
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661267
Wilkinson
Analysis of ligation and DNA b ...
Escherichia coli
Biochim. Biophys. Acta
1749
113-122
2005
-
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1
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2
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1
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1
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662343
Zhu
Structure-guided mutational an ...
Escherichia coli
J. Biol. Chem.
280
12137-12144
2005
-
-
-
-
30
-
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3
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1
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30
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1
-
-
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-
-
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-
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-
662399
Srivastava
NAD+-dependent DNA ligase (Rv3 ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
280
30273-30281
2005
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-
1
1
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5
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9
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1
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1
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5
5
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1
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660985
Gul
Staphylococcus aureus DNA liga ...
Staphylococcus aureus
Biochem. J.
383
551-559
2004
-
-
1
-
-
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2
2
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1
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1
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1
1
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2
2
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2
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1
-
-
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-
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661092
Feng
Effects of deletion and site-d ...
Thermus sp. AK16D
Biochemistry
43
12648-12659
2004
-
-
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12
-
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2
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12
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-
661248
Lu
Unique ligation properties of ...
Melanoplus sanguinipes
Biochim. Biophys. Acta
1701
37-48
2004
-
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1
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2
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2
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1
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1
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1
1
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1
2
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2
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2
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-
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1
-
1
1
-
1
1
1
-
-
-
-
-
-
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-
661350
Georlette
Adenylation-dependent conforma ...
Thermus scotoductus
Biophys. J.
86
1089-1104
2004
-
-
-
-
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1
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3
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661789
Jeon
Mutational analyses of the the ...
Thermus filiformis
FEMS Microbiol. Lett.
237
111-118
2004
-
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1
1
1
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3
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3
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1
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1
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3
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1
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1
1
2
-
2
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-
662211
Gong
Biochemical and genetic analys ...
Mycobacterium tuberculosis
J. Biol. Chem.
279
20594-20606
2004
-
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1
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1
1
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1
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4
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1
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2
1
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1
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1
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1
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1
1
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1
1
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1
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1
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1
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2
1
-
-
-
1
-
-
-
-
-
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-
-
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-
662744
Muerhoff
A non-isotopic method for the ...
Thermus thermophilus HB8
J. Virol. Methods
119
171-176
2004
-
-
-
-
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-
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2
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1
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1
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-
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-
-
-
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-
-
-
-
662787
Lavesa-Curto
Characterization of a temperat ...
Escherichia coli, Escherichia coli DSM 3757
Microbiology
150
4171-4180
2004
-
-
1
-
1
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2
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1
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2
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1
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1
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1
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2
-
-
-
-
-
-
-
-
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-
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-
663403
Gajiwala
Structural rearrangement accom ...
Enterococcus faecalis
Structure
12
1449-1459
2004
-
-
1
1
-
-
-
-
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-
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
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652463
Brotz-Oesterhelt
Specific and potent inhibition ...
Escherichia coli
J. Biol. Chem.
278
39435-39442
2003
-
1
-
-
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1
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1
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5
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1
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2
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1
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1
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1
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1
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1
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2
-
-
-
-
-
-
-
-
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-
-
652489
Georlette
Cofactor Binding modulates the ...
Escherichia coli, Thermus scotoductus
J. Biol. Chem.
278
49945-49953
2003
-
2
2
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-
-
-
-
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2
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2
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2
2
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4
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2
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2
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2
2
2
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2
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2
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4
-
-
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2
-
-
-
-
-
-
-
-
-
-
-
653853
Wilkinson
NAD+-dependent DNA ligases of ...
Escherichia coli, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Proteins Struct. Funct. Genet.
51
321-326
2003
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3
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-
-
-
-
-
-
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5
-
209
-
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3
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10
-
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3
-
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3
3
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5
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3
-
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-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650937
Cao
-
DNA ligases: Structure, functi ...
Amsacta moorei entomopoxvirus, Aquifex aeolicus, Bacillus subtilis, Melanoplus sanguinipes
Curr. Org. Chem.
6
827-839
2002
-
-
-
-
-
-
-
-
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2
-
4
-
4
-
-
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4
-
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8
-
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4
-
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4
-
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2
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4
-
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-
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-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652360
Sriskanda
Conserved residues in domain I ...
Escherichia coli
J. Biol. Chem.
277
9695-9700
2002
-
1
1
-
15
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
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2
-
-
-
-
-
-
-
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1
-
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-
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1
1
1
-
15
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649444
Lim
Mutational analyses of Aquifex ...
Aquifex pyrophilus
Arch. Biochem. Biophys.
388
253-260
2001
-
-
1
-
7
-
-
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
7
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651212
Lim
Molecular cloning and characte ...
Aquifex pyrophilus, Escherichia coli, Thermus aquaticus
Extremophiles
5
161-168
2001
4
-
1
-
-
-
-
-
-
9
1
3
-
3
-
-
1
3
-
-
-
-
6
-
3
3
3
-
1
-
-
3
-
-
-
4
-
1
3
-
-
-
-
-
-
-
-
9
1
3
-
-
-
1
-
-
-
-
6
-
3
3
3
-
1
-
-
-
-
-
-
-
-
-
651683
Kaczmarek
Cloning and functional charact ...
Staphylococcus aureus
J. Bacteriol.
183
3016-3024
2001
-
-
1
-
3
-
-
-
-
-
1
1
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6
-
-
1
1
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
652175
Sriskanda
NAD+-dependent DNA ligase enco ...
Amsacta moorei entomopoxvirus
J. Biol. Chem.
276
36100-36109
2001
-
-
1
-
10
-
-
1
-
4
1
1
-
3
-
-
1
1
-
-
-
1
2
1
-
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
10
-
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
1
2
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
653388
Sriskanda
A second NAD+-dependent DNA li ...
Escherichia coli
Nucleic Acids Res.
29
4930-4934
2001
-
-
1
-
1
-
1
-
-
4
-
1
-
3
-
-
1
1
-
-
-
1
2
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
4
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
649250
Lee
-
Crystallization and preliminar ...
Thermus filiformis
Acta Crystallogr. Sect. D
D56
357-358
2000
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650967
Lee
Crystal structure of NAD+-depe ...
Thermus filiformis
EMBO J.
19
1119-1129
2000
-
1
1
1
-
-
-
-
-
2
1
1
-
2
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651073
Georlette
A DNA ligase from the psychrop ...
Escherichia coli, Pseudoalteromonas haloplanktis, Thermus scotoductus
Eur. J. Biochem.
267
3502-3512
2000
2
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1
-
-
-
-
8
-
4
2
3
-
3
-
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1
3
-
-
-
1
6
-
-
-
-
8
1
1
-
3
-
-
-
2
-
1
3
-
-
-
-
-
-
8
-
4
2
3
-
-
-
1
-
-
-
1
6
-
-
-
-
8
1
1
-
-
-
-
-
-
-
-
653379
Tong
Ligation reaction specificitie ...
Aquifex aeolicus
Nucleic Acids Res.
28
1447-1454
2000
1
-
1
-
-
-
-
1
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4
-
1
-
6
-
-
1
1
-
-
-
-
2
-
-
-
-
1
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
4
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
652786
Timson
Functional domains of an NAD+- ...
Geobacillus stearothermophilus
J. Mol. Biol.
285
73-83
1999
-
-
1
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2
-
-
-
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1
4
1
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3
-
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1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
1
4
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653376
Sriskanda
Mutational analysis of Escheri ...
Escherichia coli
Nucleic Acids Res.
27
3953-3963
1999
-
-
1
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13
-
-
3
-
1
1
1
-
1
-
-
1
1
-
-
-
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