Application | Comment | Organism |
---|---|---|
medicine | potential target for antibiotics | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D32A | traces of nick joining activity | Escherichia coli |
D32E | traces of nick joining activity | Escherichia coli |
D32N | 9% of nick joining activity, strongly reduced self-adenylation | Escherichia coli |
D36A | traces of nick joining activity | Escherichia coli |
D36E | 4% of nick joining activity, strongly reduced self-adenylation | Escherichia coli |
D36N | 12% of nick joining activity, strongly reduced self-adenylation | Escherichia coli |
DELTA1-38 | nick joining activity abolished, no self adenylation, active with pre-adenylated nick | Escherichia coli |
DELTA1-78 | nick joining activity abolished, no self adenylation, active with pre-adenylated nick | Escherichia coli |
E10A | 90% of nick joining activity | Escherichia coli |
H23A | 10% of nick joining activity | Escherichia coli |
H23Y | 88% of nick joining activity | Escherichia coli |
Y22A | traces of nick joining activity, strongly reduced self-adenylation | Escherichia coli |
Y22S | 9% of nick joining activity | Escherichia coli |
Y35A | 2% of nick joining activity, strongly reduced self-adenylation | Escherichia coli |
Y35S | 23% of nick joining activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Escherichia coli | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Escherichia coli |