Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.2 extracted from

  • Sriskanda, V.; Shuman, S.
    Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+ (2002), J. Biol. Chem., 277, 9695-9700.
    View publication on PubMed

Application

Application Comment Organism
medicine potential target for antibiotics Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
D32A traces of nick joining activity Escherichia coli
D32E traces of nick joining activity Escherichia coli
D32N 9% of nick joining activity, strongly reduced self-adenylation Escherichia coli
D36A traces of nick joining activity Escherichia coli
D36E 4% of nick joining activity, strongly reduced self-adenylation Escherichia coli
D36N 12% of nick joining activity, strongly reduced self-adenylation Escherichia coli
DELTA1-38 nick joining activity abolished, no self adenylation, active with pre-adenylated nick Escherichia coli
DELTA1-78 nick joining activity abolished, no self adenylation, active with pre-adenylated nick Escherichia coli
E10A 90% of nick joining activity Escherichia coli
H23A 10% of nick joining activity Escherichia coli
H23Y 88% of nick joining activity Escherichia coli
Y22A traces of nick joining activity, strongly reduced self-adenylation Escherichia coli
Y22S 9% of nick joining activity Escherichia coli
Y35A 2% of nick joining activity, strongly reduced self-adenylation Escherichia coli
Y35S 23% of nick joining activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Escherichia coli DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Cofactor

Cofactor Comment Organism Structure
NAD+ required Escherichia coli