Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | 0.025 mg/ml, stimulating | Pseudoalteromonas haloplanktis | |
dithiothreitol | 10 mM, stimulating | Pseudoalteromonas haloplanktis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudoalteromonas haloplanktis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00017 | - |
nicked DNA | 4°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.00018 | - |
nicked DNA | 18°C, pH 7.8 | Escherichia coli | |
0.00024 | - |
nicked DNA | 45°C, pH 7.8 | Thermus scotoductus | |
0.0003 | - |
nicked DNA | 18°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.00047 | - |
nicked DNA | 60°C, pH 7.8 | Thermus scotoductus | |
0.00063 | - |
nicked DNA | 25°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.0007 | - |
nicked DNA | 30°C, pH 7.8 | Escherichia coli | |
0.00204 | - |
nicked DNA | 45°C, pH 7.8 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 25 mM, stimulates activity | Pseudoalteromonas haloplanktis | |
Mg2+ | required | Pseudoalteromonas haloplanktis | |
NH4+ | 10 mM, stimulates activity | Pseudoalteromonas haloplanktis | |
Zn2+ | 0.24 mol per mol protein | Pseudoalteromonas haloplanktis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74260 | - |
Q-TOF-MS-mass spectrometry, non-adenylated form | Pseudoalteromonas haloplanktis |
74590 | - |
Q-TOF-mass spectrometry, adenylated form | Pseudoalteromonas haloplanktis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Escherichia coli | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermus scotoductus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Pseudoalteromonas haloplanktis | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudoalteromonas haloplanktis | Q9RBF2 | - |
- |
Thermus scotoductus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Pseudoalteromonas haloplanktis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Escherichia coli | |
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Thermus scotoductus | |
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Pseudoalteromonas haloplanktis |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris/HCl, pH 7.1, 50 mM NaCl, 0.1 mM EDTA, 50% glycerol | Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicked DNA | - |
Escherichia coli | AMP + diphosphate + ? | - |
? | |
ATP + nicked DNA | - |
Thermus scotoductus | AMP + diphosphate + ? | - |
? | |
ATP + nicked DNA | - |
Pseudoalteromonas haloplanktis | AMP + diphosphate + ? | - |
? | |
ATP + nicked DNA | - |
Escherichia coli | ? | - |
? | |
ATP + nicked DNA | - |
Thermus scotoductus | ? | - |
? | |
ATP + nicked DNA | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermus scotoductus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Pseudoalteromonas haloplanktis | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Thermus scotoductus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Pseudoalteromonas haloplanktis | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
nicked DNA | 4°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.016 | - |
nicked DNA | 45°C, pH 7.8 | Escherichia coli | |
0.021 | - |
nicked DNA | 18°C, pH 7.8 | Escherichia coli | |
0.03 | - |
nicked DNA | 30°C, pH 7.8 | Escherichia coli | |
0.034 | - |
nicked DNA | 18°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.047 | - |
nicked DNA | 25°C, pH 7.8 | Pseudoalteromonas haloplanktis | |
0.053 | - |
nicked DNA | 45°C, pH 7.8 | Thermus scotoductus | |
0.132 | - |
nicked DNA | 60°C, pH 7.8 | Thermus scotoductus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Pseudoalteromonas haloplanktis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | very low activity above or below this range | Pseudoalteromonas haloplanktis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Escherichia coli | |
NAD+ | required | Thermus scotoductus | |
NAD+ | required | Pseudoalteromonas haloplanktis |