Cloned (Comment) | Organism |
---|---|
full length and truncated enzymes expressed in Escherichia coli BL21 (DE3) | Aquifex pyrophilus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-525 | no DNA-binding | Aquifex pyrophilus |
DELTA1-90 | very low self adenylation activity, active in DNA-ligation, reduced DNA-binding | Aquifex pyrophilus |
DELTA1-90,363-719 | no self adenylation | Aquifex pyrophilus |
DELTA1-90,487-719 | very low self adenylation activity | Aquifex pyrophilus |
DELTA363-719 | full self adenylation activity | Aquifex pyrophilus |
DELTA517-719 | full self adenylation activity | Aquifex pyrophilus |
DELTA636-719 | active in DNA-ligation | Aquifex pyrophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc binding motif, Cys437, Cys440, Cys455, Cys460 | Aquifex pyrophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Aquifex pyrophilus | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex pyrophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins | Aquifex pyrophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide | 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond | Aquifex pyrophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Aquifex pyrophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA | Aquifex pyrophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Aquifex pyrophilus |