Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na+ | above 20-150 mM | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000185 | - |
NAD+ | - |
Thermus thermophilus | |
0.000025 | - |
5'-phosphoryl termini of poly(dA-dT) | - |
Thermus thermophilus | |
0.14 | - |
(dT)10 | - |
Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | activates at 1-150 mM | Thermus thermophilus | |
Mg2+ | requires divalent cations, Mn2+ or Mg2+ | Thermus thermophilus | |
Mg2+ | optimal concentration: 5-9 mM | Thermus thermophilus | |
Mn2+ | requires divalent cations, Mn2+ or Mg2+ | Thermus thermophilus | |
Mn2+ | optimal concentration: 3-6 mM | Thermus thermophilus | |
NH4+ | activates at 1-150 mM | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78500 | - |
1 * 78500, SDS-PAGE | Thermus thermophilus |
80400 | - |
sedimentation equilibrium ultracentrifugation | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermus thermophilus |
Storage Stability | Organism |
---|---|
4°C, stable for several months | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (dT)10 | - |
Thermus thermophilus | ? | - |
? | |
ATP + 5'-phosphoryl termini of poly(dA-dT) | - |
Thermus thermophilus | ? | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | (dT)8, (dT)10, (dT)12-18 | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | cohesive-end DNA restriction fragments | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? | |
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | nick-closing activity | Thermus thermophilus | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 78500, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 37 | cohesive-end DNA restriction fragments | Thermus thermophilus |
27.7 | - |
p(dT)8, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
34.5 | - |
p(dT)10, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
37 | - |
p(dT)12-18, joining of thymidylate oligomers in the presence of poly(dA) as a template | Thermus thermophilus |
65 | 72 | nick-closing activity | Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 85 | nick-closing activity observed in the range | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
activity decreases remarkably below 4°C | Thermus thermophilus |
24 | - |
stable for 1 week | Thermus thermophilus |
37 | - |
stable for 1 week | Thermus thermophilus |
65 | - |
2 days, 50% loss of activity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | Tris-HCl buffer | Thermus thermophilus |
8 | - |
collidine/HCl buffer | Thermus thermophilus |