Literature summary for 6.5.1.2 extracted from

Takahashi, M.; Uchida, T.
Thermophilic HB8 DNA ligase: effects of polyethylene glycols and polyamines on blunt-end ligation of DNA (1986), J. Biochem., 100, 123-131.

Search for more literature for 6.5.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
polyamines	blunt-end ligation are linear oligomers. Optimal concentration: caldopentamine, 0.05 mM, thermine, 0.1-0.2 mM, spermine, 0.2 mM, thermospermine, 0.4 mM, sperminediol, 0.75 mM	Thermus thermophilus
Polyethylene glycol	in presence of high molecular weight polyethylene glycol 20000, 6000 or 1000, 8-28%, the enzyme catalyzes blunt-end intermolecular joining to yield linear oligomers, but no circular DNA forms. In presence of low molecular polyethylene glycols 400, 200, 8-80%, or the monomer ethylene glycol, 16-80%, the circular forms formed by intramolecular ligation are also detected	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	blunt-end ligation, intermolecular joining to yield linear oligomers, but no circular DNA forms in presence of high concentrations of polyethylene glycols	Thermus thermophilus	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	blunt-end ligation	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
72	-	2 h, stable below, destabilized above	Thermus thermophilus

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Release 2023.1 (January 2023)