Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.1 extracted from

  • de Ory, A.; Nagler, K.; Carrasco, B.; Raguse, M.; Zafra, O.; Moeller, R.; de Vega, M.
    Identification of a conserved 5'-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair (2016), Nucleic Acids Res., 44, 1833-1844 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Bacillus subtilis
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Pseudomonas aeruginosa
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine Bacillus subtilis
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine Pseudomonas aeruginosa
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus subtilis
Ni-NTA column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Bacillus subtilis (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Pseudomonas aeruginosa (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine
-
 Bacillus subtilis [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine
-
 Pseudomonas aeruginosa [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?
additional information the enzyme also has an intrinsic 5'-2-deoxyribose-5-phosphate lyase activity located at the N-terminal ligase domain that in coordination with the polymerization and ligase activities allows efficient repairing of 2'-deoxyuridine-containing DNA in an in vitro reconstituted base excision repair reaction Bacillus subtilis ?
-
 ?
additional information the enzyme also has an intrinsic 5'-2-deoxyribose-5-phosphate lyase activity located at the N-terminal ligase domain that in coordination with the polymerization and ligase activities allows efficient repairing of 2'-deoxyuridine-containing DNA in an in vitro reconstituted base excision repair reaction Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
ATP-dependent DNA ligase
-
 Bacillus subtilis
ATP-dependent DNA ligase
-
 Pseudomonas aeruginosa
DNA repair ligase D
-
 Bacillus subtilis
DNA repair ligase D
-
 Pseudomonas aeruginosa
LigD
-
 Bacillus subtilis
LigD
-
 Pseudomonas aeruginosa