Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami (DE3) cells | Thermococcus sp. 1519 |
Protein Variants | Comment | Organism |
---|---|---|
A287K | the mutant enzyme has a half-life time at 94°C of 20 min | Thermococcus sp. 1519 |
A387K | the mutant enzyme has a half-life time at 94°C of 15 min | Thermococcus sp. 1519 |
G304D | the mutant enzyme has a half-life time at 94°C of 25 min | Thermococcus sp. 1519 |
S364I | the mutant enzyme has a half-life time at 94°C of 20 min | Thermococcus sp. 1519 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m | Thermococcus sp. 1519 | - |
(deoxyribonucleotide)n+m + AMP + diphosphate | - |
? | |
ATP + [DNA ligase]-L-lysine | Thermococcus sp. 1519 | - |
[DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus sp. 1519 | C0LJI8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, and Superdex 200 gel filtration | Thermococcus sp. 1519 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m | - |
Thermococcus sp. 1519 | (deoxyribonucleotide)n+m + AMP + diphosphate | - |
? | |
ATP + [DNA ligase]-L-lysine | - |
Thermococcus sp. 1519 | [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63380, calculated from amino acid sequence | Thermococcus sp. 1519 |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent DNA ligase | - |
Thermococcus sp. 1519 |
Lig | - |
Thermococcus sp. 1519 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | - |
the unmodified enzyme loses half of its activity upon 8 min incubation at 94°C | Thermococcus sp. 1519 |