Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 100 mM, approximately 70% inhibition | Aeropyrum pernix | |
NaCl | 100 mM, approximately 70% inhibition | Aeropyrum pernix | |
NH4Cl | 100 mM, approximately 70% inhibition | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
Co2+ | the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
KCl | optimum concentration: 5 mM | Aeropyrum pernix | |
Mg2+ | optimal concentration: 15 mM, the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix | |
Mn2+ | optimal concentration: 7.5 mM, the enzyme requires Mg2+ or Mn2+ for activity, Ca2+ or Co2+ are less effective | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
1 * 69000, SDS-PAGE | Aeropyrum pernix |
69196 | - |
1 * 69196, the authors refer to the protein of 619 amino acids that is later reannotated as a protein of 602 amino acids (67747.6 Da), calculated from sequence | Aeropyrum pernix |
70000 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YD18 | the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein | - |
Aeropyrum pernix DSM 11879 | Q9YD18 | the ApeLig gene is originally annotated as a protein of 619 amino acids, with a calculated mass of 69196.2 Da. Later it was reannotated as a protein of 602 amino acids (67747.6 Da), in which 17 amino acids are truncated from the N-terminus of the originally annotated protein. The UniProt-number refers to the 602 amino acid protein | - |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | activity with ADP is slightly lower than with ATP | Aeropyrum pernix | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
ADP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | activity with ADP is slightly lower than with ATP | Aeropyrum pernix DSM 11879 | AMP + phosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme is inactive when ATP was substituted by AMP or NAD+ | Aeropyrum pernix | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | the enzyme is inactive when ATP was substituted by AMP or NAD+ | Aeropyrum pernix DSM 11879 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 69000, SDS-PAGE | Aeropyrum pernix |
monomer | 1 * 69196, the authors refer to the protein of 619 amino acids that is later reannotated as a protein of 602 amino acids (67747.6 Da), calculated from sequence | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
APE1094 | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
optimum for nick-closing activity is above 70°C | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | in the nick-closing assay, the recombinant ligase shows more than 80% activity in the temperature range 65-80°C. Ligase activity declines at temperatures below 35°C. A drastic decrease in activity is observed between 80 and 90°C | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
stable for 60 min, half-life: 25 min | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
optimum for nick-closing activity | Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.2 | 80% of maximal activity is observed between pH 7.0 and 8.2 | Aeropyrum pernix |