Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BW23474 cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H373A | mutant without 3'-5' single-stranded DNA exonuclease activity | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese | Mycobacterium tuberculosis | |
Mn2+ | LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Mycobacterium tuberculosis | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
additional information | the PolDom domain of LigD is required for efficient and accurate DNA repair and increases deletions in re-circularized plasmid DNA when expressed with Ku protein in Escherichia coli. The LigDom domain of LigD can function in Ku-dependent repair | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 100000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
adenosine triphosphate-dependent ligase | - |
Mycobacterium tuberculosis |
ATP-dependent ligase | - |
Mycobacterium tuberculosis |
ligase D | - |
Mycobacterium tuberculosis |
LigD | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
Organism | Comment | Expression |
---|---|---|
Mycobacterium tuberculosis | LigD protein is only expressed when 0.2% L-arabinose is added to the culture | up |