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Literature summary for 6.5.1.1 extracted from

  • Wright, D.; DeBeaux, A.; Shi, R.; Doherty, A.J.; Harrison, L.
    Characterization of the roles of the catalytic domains of Mycobacterium tuberculosis ligase D in Ku-dependent error-prone DNA end joining (2010), Mutagenesis, 25, 473-481.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BW23474 cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
H373A mutant without 3'-5' single-stranded DNA exonuclease activity Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese Mycobacterium tuberculosis
Mn2+ LigD has 3'-5' single-stranded DNA exonuclease activity that requires magnesium or manganese Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
additional information the PolDom domain of LigD is required for efficient and accurate DNA repair and increases deletions in re-circularized plasmid DNA when expressed with Ku protein in Escherichia coli. The LigDom domain of LigD can function in Ku-dependent repair Mycobacterium tuberculosis ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 100000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
adenosine triphosphate-dependent ligase
-
 Mycobacterium tuberculosis
ATP-dependent ligase
-
 Mycobacterium tuberculosis
ligase D
-
 Mycobacterium tuberculosis
LigD
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis LigD protein is only expressed when 0.2% L-arabinose is added to the culture up