Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
LigIII in complex with 22-mer nicked DNA, hanging drop vapor diffusion method, using 1.8 M ammonium sulfate and 0.1 M sodium acetate (pH 5.6) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K323E | the mutant has significantly reduced blunt end DNA ligation activity (87% decrease in the initial velocity of blunt end DNA ligation compared to wild type enzyme), but has very little effect on DNA nick joining activity, the mutation selectively blocks zinc finger function | Homo sapiens |
K323E/E265K | the mutations do not restore efficient blunt end DNA joining activity | Homo sapiens |
R327E | the mutant is nearly devoid of blunt end joining activity, mimicking a deletion of the zinc finger | Homo sapiens |
R327E/D262R | the mutations do not restore efficient blunt end DNA joining activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains a PARP-like zinc finger | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase III | - |
Homo sapiens |
LigIII | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | besides repairing nuclear DNA, ligase III is the only mitochondrial DNA ligase in which it functions in DNA repair and replication | Homo sapiens |