Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta2(DE3)pLysS cells | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
ATP-dependent DNA ligase in the DNA-unbound unadenylated state, hanging drop vapor diffusion method, using 100 mM Tris-HCl pH 9.0, 0.4 M sodium dihydrogen phosphate, 1.2 M dipotassium hydrogen phosphate, and 10 mM magnesium chloride, at 4°C | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Archaeoglobus fulgidus | - |
AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29632 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, Source 15Q ion-exchange column chromatography, and Superdex-200 gel filtration | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Archaeoglobus fulgidus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent DNA ligase | - |
Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Archaeoglobus fulgidus |