Cloned (Comment) | Organism |
---|---|
expression of His-tagged DNA ligase I in Escherichia coli strain BL21(DE3) | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Sulfurisphaera tokodaii | |
PCNA | inhibition of the DNA ligase I activity by proliferating cell nuclear antigen, PCNA, complexes StoPCNA. StoPCNA1 and StoPCNA3, overview | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Sulfurisphaera tokodaii |
Organic Solvent | Comment | Organism |
---|---|---|
formamide | inactivation at about 50% | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q976G4 | - |
- |
Sulfurisphaera tokodaii 7 | Q976G4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DNA ligase I from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + DNA substrate S4 | - |
Sulfurisphaera tokodaii | AMP + diphosphate + ? | - |
? | |
ATP + DNA substrate S4 | - |
Sulfurisphaera tokodaii 7 | AMP + diphosphate + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase I | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfurisphaera tokodaii |