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Literature summary for 6.5.1.1 extracted from

  • Zhu, H.; Wang, L.K.; Shuman, S.
    Essential constituents of the 3'-phosphoesterase domain of bacterial DNA ligase D, a nonhomologous end-joining enzyme (2005), J. Biol. Chem., 280, 33707-33715.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D15A 3'-ribonuclease activity with D10R2 primer-template is 110% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 3.3% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 4.9% of wild-type activity Pseudomonas aeruginosa
D83A 3'-ribonuclease activity with D10R2 primer-template is 57% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 51% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 70% of wild-type activity Pseudomonas aeruginosa
E21A 3'-ribonuclease activity with D10R2 primer-template is 130% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is less than 0.1% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is less than 0.1% of wild-type activity Pseudomonas aeruginosa
H42A 3'-ribonuclease activity with D10R2 primer-template is less than 0.1% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is less than 0.1% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 0.2% of wild-type activity Pseudomonas aeruginosa
H48A 3'-ribonuclease activity with D10R2 primer-template is less than 0.1% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 0.8% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 0.2% of wild-type activity Pseudomonas aeruginosa
K66A 3'-ribonuclease activity with D10R2 primer-template is 2.9% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 28% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 48% of wild-type activity Pseudomonas aeruginosa
Q40A 3'-ribonuclease activity with D10R2 primer-template is 41% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 6.7% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 8.6% of wild-type activity Pseudomonas aeruginosa
R14A 3'-ribonuclease activity with D10R2 primer-template is 77% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 0.2% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 0.1% of wild-type activity Pseudomonas aeruginosa
R46A 3'-ribonuclease activity with D10R2 primer-template is 16% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 19% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 67% of wild-type activity Pseudomonas aeruginosa
R76A 3'-ribonuclease activity with D10R2 primer-template is 1.1% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 16% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 44% of wild-type activity Pseudomonas aeruginosa
Y88A 3'-ribonuclease activity with D10R2 primer-template is 7.1% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 0.2% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is less than 0.1% of wild-type activity Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Pseudomonas aeruginosa catalyzes end-healing and end-sealing steps during nonhomologous end joining AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m catalyzes end-healing and end-sealing steps during nonhomologous end joining Pseudomonas aeruginosa AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Synonyms

Synonyms Comment Organism
DNA ligase D
-
 Pseudomonas aeruginosa
LigD
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 Pseudomonas aeruginosa