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Literature summary for 6.5.1.1 extracted from

  • Gong, C.; Martins, A.; Bongiorno, P.; Glickman, M.; Shuman, S.
    Biochemical and genetic analysis of the four DNA ligases of mycobacteria (2004), J. Biol. Chem., 279, 20594-20606.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ATP-dependent ligase LigB is produced in Escherichia coli as a His10-LigB fusion protein Mycobacterium tuberculosis
ATP-dependent ligase LigC is produced in Escherichia coli as a His10-LigC fusion protein Mycobacterium tuberculosis
ATP-dependent ligase LigD is produced in Escherichia coli as a His10-LigD fusion protein Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
ATP ATP-dependent ligase LigB Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigB displays vigorous nick sealing activity in presence of NAD+ and ATP AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigC displays weak nick joining activity and generates high levels of DNA adenylate intermediate AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mycobacterium tuberculosis ATP-dependent ligase LigD displays weak nick joining activity and generates high levels of DNA adenylate intermediate AMP + diphosphate + (deoxyribonucleotide)n+m
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
ATP-dependent ligase LigB
-
Mycobacterium tuberculosis
-
ATP-dependent ligase LigC
-
Mycobacterium tuberculosis
-
ATP-dependent ligase LigD
-

Purification (Commentary)

Purification (Comment) Organism
His10-LigB fusion protein expressed in Escherichia coli Mycobacterium tuberculosis
His10-LigC fusion protein expressed in Escherichia coli Mycobacterium tuberculosis
His10-LigD fusion protein expressed in Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigB displays vigorous nick sealing activity in presence of NAD+ and ATP Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigC displays weak nick joining activity and generates high levels of DNA adenylate intermediate Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ATP-dependent ligase LigD displays weak nick joining activity and generates high levels of DNA adenylate intermediate Mycobacterium tuberculosis AMP + diphosphate + (deoxyribonucleotide)n+m
-
?

Subunits

Subunits Comment Organism
monomer ATP-dependent ligase LigB Mycobacterium tuberculosis
monomer ATP-dependent ligase LigC Mycobacterium tuberculosis
monomer ATP-dependent ligase LigD Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
ATP-dependent ligase LigB
-
Mycobacterium tuberculosis
ATP-dependent ligase LigC
-
Mycobacterium tuberculosis
ATP-dependent ligase LigD
-
Mycobacterium tuberculosis
LigB
-
Mycobacterium tuberculosis
LigC
-
Mycobacterium tuberculosis
LigD
-
Mycobacterium tuberculosis
MtuLigB
-
Mycobacterium tuberculosis
MtuLigC
-
Mycobacterium tuberculosis
MtuLigD
-
Mycobacterium tuberculosis