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Literature summary for 6.5.1.1 extracted from

  • Nakatani, M.; Ezaki, S.; Atomi, H.; Imanaka, T.
    A DNA ligase from a hyperthermophilic archaeon with unique cofactor specificity (2000), J. Bacteriol., 182, 6424-6433.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cations stimulate activity, maximal activation at 10-30 mM Thermococcus kodakarensis
Mg2+ required for activity, maximal activity at 14-18 mM Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52100
-
gel filtration Thermococcus kodakarensis
64079
-
1 * 64079, deduced from nucleotide sequence Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermococcus kodakarensis
-
AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q9HHC4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant ligTK, ResourceQ, ResourceS, Superdex-200 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)30 + (deoxyribonucleotide)40 ligTK displays little but significant ativity with NAD+ Thermococcus kodakarensis AMP + diphosphate + (deoxyribonucleotide)70
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Thermococcus kodakarensis AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64079, deduced from nucleotide sequence Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
ligTK
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
at 0.00002 mM ligTK Thermococcus kodakarensis
70 80 at 0.0002 mM ligTK Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 at 0.0002 mM ligTK Thermococcus kodakarensis
35 80 at 0.00002 mM ligTK Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thermococcus kodakarensis