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Literature summary for 6.5.1.1 extracted from

  • Cao, W.
    DNA ligases: Structure, function and mechanism (2002), Curr. Org. Chem., 6, 827-839.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia phage T7
2.0 A crystal structure of D29A mutant complexed with AMP Chlorella virus

Protein Variants

Protein Variants Comment Organism
D29A crystal structure Chlorella virus
K159L adenylation site mutant, aboragates the ability of the ligase to covalently link to an AMP moiety Escherichia phage T7
K29A arrests ligation reaction at the substrate adenylation step Chlorella virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Vaccinia virus
Mn2+ required for activity Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Vaccinia virus
-
AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Escherichia phage T7
-
AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Chlorella virus
-
AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Organism

Organism UniProt Comment Textmining
Chlorella virus
-
-
-
Escherichia phage T7
-
-
-
Vaccinia virus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Vaccinia virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Escherichia phage T7 AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Chlorella virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m both nick sealing and blunt end ligation Escherichia phage T7 AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m joins 2 DNA sequences on a DNA template but not on a RNA template Chlorella virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m ligates sticky end substrates efficiently but requires 10% polyethylene glycol 8000 for blunt end ligation Vaccinia virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Synonyms

Synonyms Comment Organism
T4 ATP ligase
-
Escherichia phage T7
Vaccinia ligase
-
Vaccinia virus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
significant loss of ligation activity Vaccinia virus