Crystallization (Comment) | Organism |
---|---|
- |
Escherichia phage T7 |
2.0 A crystal structure of D29A mutant complexed with AMP | Chlorella virus |
Protein Variants | Comment | Organism |
---|---|---|
D29A | crystal structure | Chlorella virus |
K159L | adenylation site mutant, aboragates the ability of the ligase to covalently link to an AMP moiety | Escherichia phage T7 |
K29A | arrests ligation reaction at the substrate adenylation step | Chlorella virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Vaccinia virus | |
Mn2+ | required for activity | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Vaccinia virus | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Escherichia phage T7 | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Chlorella virus | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus | - |
- |
- |
Escherichia phage T7 | - |
- |
- |
Vaccinia virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Vaccinia virus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Escherichia phage T7 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Chlorella virus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | both nick sealing and blunt end ligation | Escherichia phage T7 | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | joins 2 DNA sequences on a DNA template but not on a RNA template | Chlorella virus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | ligates sticky end substrates efficiently but requires 10% polyethylene glycol 8000 for blunt end ligation | Vaccinia virus | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Synonyms | Comment | Organism |
---|---|---|
T4 ATP ligase | - |
Escherichia phage T7 |
Vaccinia ligase | - |
Vaccinia virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
significant loss of ligation activity | Vaccinia virus |