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Literature summary for 6.5.1.1 extracted from

  • Hayashi, K.; Nakazawa, M.; Ishizaki, Y.; Obayashi, A.
    Influence of monovalent cations on the activity of T4 DNA ligase in the presence of polyethylene glycol (1985), Nucleic Acids Res., 13, 3261-3271.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
Polyethylene glycol polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
K+ extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate Tequatrovirus T4
Na+ extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate. Resistance to inhibition in the order from strong to weak: HindIII, PstI, EcoRI, BamHI, SalI in cohesive end ligation, and EcoRV, ScaI, PvuII, NruI in blunt end ligation Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
K+ polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4
Na+ polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation is increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intamolecular ligation occurs at low salt concentrations, 0.100 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10 16 when polyethylene glycol is not present Tequatrovirus T4
37
-
 with 10% polyethylene glycol 6000 intermolecular ligation at high concentrations of salt is stimulated by raising the temperature from 19°C to 37°C Tequatrovirus T4