Activating Compound | Comment | Organism | Structure |
---|---|---|---|
XRCC1 | DNA ligase III-alpha forms a complex with the DNA single-strand break repair protein XRCC1 | Mammalia |
Cloned (Comment) | Organism |
---|---|
DNA ligase I, III-alpha, III-beta, IV | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibits the last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation | Mammalia | |
pyridoxal 5'-phosphate | inhibits last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation | Mammalia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Drosophila melanogaster | 5634 | - |
nucleus | - |
Mammalia | 5634 | - |
nucleus | - |
Homo sapiens | 5634 | - |
nucleus | - |
Bos taurus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
x * 125000, denaturing PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Drosophila melanogaster | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Homo sapiens | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Saccharomyces cerevisiae | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Bos taurus | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Vaccinia virus | - |
? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Mammalia | DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Mammalia | DNA ligase I is involved in DNA replication | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Homo sapiens | - |
- |
- |
Mammalia | - |
DNA ligase I, II, III-alpha, III-beta, and IV. DNA ligase II appears to be derived from DNA ligase III by a proteolytic mechanism | - |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Vaccinia virus | - |
- |
- |
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DNA ligase I | Bos taurus |
DNA ligase I, II, III, and IV | Mammalia |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate | mechanism: 1. formation of a covalent enzyme-adenylate complex, the ATP is cleaved to AMP and diphosphate with adenylyl residue linked by a phosphoramidate bond to the epsilon-amino group of a specific lysine residue at the active site of the protein, 2. the activated AMP residue of the DNA ligase-adenylate intermediate is transferred to the 5'-phosphate terminus of a single strand break in double-stranded DNA to generate a covalent DNA-AMP complex with a 5'-5' phosphoanhydride bond, 3. in the final step of DNA ligation, unadenylated DNA ligase is required for the generation of a phosphodiester bond and catalyzes displacement of the AMP residue through attack by adjacent 3'-hydroxyl group of the adenylylated site | Mammalia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ | male meiotic cell, ligase III-beta is expressed only in male meiotic germ cells | Mammalia | - |
testis | - |
Homo sapiens | - |
testis | - |
Bos taurus | - |
thymus | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Drosophila melanogaster | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Mammalia | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharomyces cerevisiae | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Xenopus laevis | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Schizosaccharomyces pombe | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Vaccinia virus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Drosophila melanogaster | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Homo sapiens | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharomyces cerevisiae | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Bos taurus | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Vaccinia virus | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination | Mammalia | ? | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase I is involved in DNA replication | Mammalia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Drosophila melanogaster |
? | - |
Homo sapiens |
? | - |
Saccharomyces cerevisiae |
? | - |
Schizosaccharomyces pombe |
? | x * 125000, denaturing PAGE | Bos taurus |