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show all sequences of 6.4.1.6

Nitrate-dependent degradation of acetone by Alicycliphilus and Paracoccus strains and comparison of acetone carboxylase enzymes

Dullius, C.H.; Chen, C.Y.; Schink, B.; Appl. Environ. Microbiol. 77, 6821-6825 (2011)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
three bands observed with SDS-PAGE: 85000, 78000, 20000 Da
Alicycliphilus denitrificans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetone + CO2 + ATP + H2O
Alicycliphilus denitrificans
-
acetoacetate + AMP + phosphate
-
-
?
butanone + CO2 + ATP + H2O
Alicycliphilus denitrificans
strain KN Bun08 also carboxylates also butanone
3-oxopentanoate + AMP + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alicycliphilus denitrificans
-
isolated from strain KN Bun08 and K601T
-
Purification (Commentary)
Commentary
Organism
enriched from cell extracts of Alicycliphilus denitrificans by two subsequent DEAE Sepharose column procedures
Alicycliphilus denitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetone + CO2 + ATP + H2O
-
726717
Alicycliphilus denitrificans
acetoacetate + AMP + phosphate
-
-
-
?
butanone + CO2 + ATP + H2O
strain KN Bun08 also carboxylates also butanone
726717
Alicycliphilus denitrificans
3-oxopentanoate + AMP + phosphate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Alicycliphilus denitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Alicycliphilus denitrificans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
three bands observed with SDS-PAGE: 85000, 78000, 20000 Da
Alicycliphilus denitrificans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetone + CO2 + ATP + H2O
Alicycliphilus denitrificans
-
acetoacetate + AMP + phosphate
-
-
?
butanone + CO2 + ATP + H2O
Alicycliphilus denitrificans
strain KN Bun08 also carboxylates also butanone
3-oxopentanoate + AMP + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
enriched from cell extracts of Alicycliphilus denitrificans by two subsequent DEAE Sepharose column procedures
Alicycliphilus denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetone + CO2 + ATP + H2O
-
726717
Alicycliphilus denitrificans
acetoacetate + AMP + phosphate
-
-
-
?
butanone + CO2 + ATP + H2O
strain KN Bun08 also carboxylates also butanone
726717
Alicycliphilus denitrificans
3-oxopentanoate + AMP + phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Alicycliphilus denitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Alicycliphilus denitrificans
Other publictions for EC 6.4.1.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2002
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5
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1
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2
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2
1
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1
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9
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7
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2
3
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9
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2
3
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1
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4
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1
1
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2
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1
1
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1
1
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1
1
10
1
1
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1
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349806
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94
8456-8461
1997
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1
3
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2
7
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1
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3
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2
7
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1
4
1
1
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1
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